지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.10
- 수록면
- 573 - 577 (5page)
이용수
초록· 키워드
오류제보하기
A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.
목차
Abstract
REFERENCES
참고문헌 (21)
참고문헌 신청
Nielsen HV / 1999 / Complete protection against lethal Toxoplasma gondii infection in mice immunized with a plasmid encoding the SAG1 gene / Infect Immun 67:6358~6363
Dubey JP / 1994 / Persistence of immunity to toxoplasmosis in pigs vaccinated with a non-persistent strain of Toxoplasma gondii / Am J Vet Res 55:982~987
Foulon W / 1999 / Prenatal diagnosis of congenital toxoplasmosis : a multicenter evaluation of different diagnostic parameters / Am J Obstet 181:843~847
Pelloux H / 1996 / A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction / FEMS Microbiol Lett 138:11~15
Wang YH / 2011 / Development of an immunochromatographic strip for the rapid detection of Toxoplasma gondii circulating antigens / Parasitol Int 60:105~107
Dubey JP / 1994 / Persistence of immunity to toxoplasmosis in pigs vaccinated with a non-persistent strain of Toxoplasma gondii / Am J Vet Res 55:982~987
Foulon W / 1999 / Prenatal diagnosis of congenital toxoplasmosis : a multicenter evaluation of different diagnostic parameters / Am J Obstet 181:843~847
Pelloux H / 1996 / A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction / FEMS Microbiol Lett 138:11~15
Wang YH / 2011 / Development of an immunochromatographic strip for the rapid detection of Toxoplasma gondii circulating antigens / Parasitol Int 60:105~107
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UCI(KEPA) : I410-ECN-0101-2014-510-003484324