The aim of this study was to investigate the mechanical properties of dental glass-ionomer cement containing titania nanofiber (TNF). The TNF was fabricated by electrospinning using chemicals and calcinated at 800℃, and mixed with a commercial glass ionomer powder by ball milling in 10 wt%. Specimens for compressive strength (φ4 ㎜ x 6 ㎜) and diametral tensile strength (φ6 ㎜ x 4 ㎜) were prepared by mixing with the cement liquid and kept in an water of (37±1)℃. Mechanical strength tests were conducted at 1 h, 24 h, and 7 days at cross-head speed of 1 ㎜/min. Data were statistically analyzed by one-way ANOVA and Tukey HSD post-hoc test. Mean strength values of GIC containing TNF was significantly higher than unmodified or ball milled groups without TNF (p<0.05). Scanning electron micrographs showed that TNF was embedded and bonded with the cement matrix. Results indicated that the TNF was considered as potential reinforcing material for dental glass-ionomer cement.