Purpose: The purpose of this study was to investigate perceived stressor, the characteristics of personality and stress responses of college students and to compare their gender differences. Method: This study was cross-sectional survey and participants were 146 college students. Data was collected by Perceived Daily Life Stressor Scale of college students, Lim’s Character Style Inventory, Vulnerability of Personality, and Stress Response Index, demographic questionnaire from May to September, 2012 in two cities. Results: Friend relationship stressor(p=.024) and professor relationship stressor(p=.032) among daily life stressors of female students were significantly higher than male students. Fatigue(p=.009), anger(p=.046), somatization(p<.001) and total stress response(p=.049) of female students were significantly higher than male student. Challenge(p=.034) of LCSI of female students was significantly higher than male student and stability(p=.018) of male students was significantly higher than female students. Regression model of stress response in male college students was significant(F=35.18, p<.001) and influencing factors were vulnerability of personality and challenge(R<SUP>2</SUP>=.544). Regression model of stress response in female college students was significant(F=39.38, p<.001) and influencing factors were vulnerability of personality, perceived stress type, and sociability(R<SUP>2</SUP>=.612). Conclusion: These findings need to be reflected to develop stress management programs and mental health promotion programs for college students.