Objectives: This study was to propose a standardized method of retention enema by extracting elements associated with published experimental methods of retention enema. Methods: 1) Literature search (1) Data sources: Retrieval was made, using ‘Enema" or "Retention Enema’ as search words in Medline, Cochrane library, KISS, RISS, and KDSL DB. (2) Study selection: Two authors excluded irrelevant papers and chose qualified abstracts from the rest of the papers. The chosen studies were reviewed fully by the authors so that they could screen the significant papers based on the exclusion criteria. (3) Data extraction: Data on the total number of subjects, the target disease, the animal species, the type, quality, length, and diameter of catheter, the insertion length of catheter, the sample, the sample volume, the process of retention enema and the number of retention enemas performed were extracted from the selected studies. 2) Performing preliminary experiments about the elements associated with experimental methods of retention enema. Results & Conclusions: The standardized experimental method is as follows: animal: 280-320g SD rat 1) inserting a rectal tube of 8 Fr that measures 8 cm into the animal"s anus, 2) injecting herbal medicines at the volume of 6 ml and 3) holding them for 10 minutes.