지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.8
- 수록면
- 355 - 365 (11page)
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초록· 키워드
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The enteric protozoan parasite Entamoeba histolytica is the causative agent of human amebiasis. During infection, adherence of E. histolytica through Gal/GalNAc lectin on the surface of the amoeba can induce caspase-3-dependent or -independent host cell death. Phosphorylinositol 3-kinase (PI3K) and protein kinase C (PKC) in E. histolytica play an important function in the adhesion, killing, or phagocytosis of target cells. In this study, we examined the role of amoebic PI3K and PKC in amoeba-induced apoptotic cell death in Jurkat T cells. When Jurkat T cells were incubated with E. histolytica trophozoites, phosphatidylserine (PS) externalization and DNA fragmentation in Jurkat cells were markedly increased compared to those of cells incubated with medium alone. However, when amoebae were pretreated with a PI3K inhibitor, wortmannin before being incubated with E. histolytica, E. histolytica-induced PS externalization and DNA fragmentation in Jurkat cells were significantly reduced compared to results for amoebae pretreated with DMSO. In addition, pretreatment of amoebae with a PKC inhibitor, staurosporine strongly inhibited Jurkat T cell death. However, E. histolytica-induced cleavage of caspase-3, -6, and -7 were not inhibited by pretreatment of amoebae with wortmannin or staurosporin. In addition, we found that amoebic PI3K and PKC have an important role on amoeba adhesion to host compartment. These results suggest that amebic PI3K and PKC activation may play an important role in caspase-independent cell death in Entamoeba-induced apoptosis.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (40)
참고문헌 신청
Huston CD / 2004 / Parasite and host contributions to the pathogenesis of amebic colitis / Trends Parasitol 20:23~26
PetriWA Jr / 2002 / The bittersweet interface of parasite and host : lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica / Annu Rev Microbiol 56:39~64
Huston CD / 2000 / Caspase 3-dependent killing of host cells by the parasite Entamoeba histolytica / Cell Microbiol 2:617~625
Huston CD / 2003 / Apoptotic killing and phagocytosis of host cells by the parasite Entamoeba histolytica / Infect Immun 71:964~972
Li E / 1988 / Use of Chinese hamster ovary cells with altered glycosylation patterns to define the carbohydrate specificity of Entamoeba histolytica adhesion / J Exp Med 167:1725~1730
PetriWA Jr / 2002 / The bittersweet interface of parasite and host : lectin-carbohydrate interactions during human invasion by the parasite Entamoeba histolytica / Annu Rev Microbiol 56:39~64
Huston CD / 2000 / Caspase 3-dependent killing of host cells by the parasite Entamoeba histolytica / Cell Microbiol 2:617~625
Huston CD / 2003 / Apoptotic killing and phagocytosis of host cells by the parasite Entamoeba histolytica / Infect Immun 71:964~972
Li E / 1988 / Use of Chinese hamster ovary cells with altered glycosylation patterns to define the carbohydrate specificity of Entamoeba histolytica adhesion / J Exp Med 167:1725~1730
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UCI(KEPA) : I410-ECN-0101-2015-510-002710538