지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.8
- 수록면
- 278 - 284 (7page)
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초록· 키워드
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We present microfluidic method to rapidly analyze the effect of temperature on the change of morphologies of Antarctic bacteria (Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp.). The microfluidic device is able to generate stable temperature gradient from 7 to 40℃ and dramatically reduce the number of experiments, experimental cost and labor, and amount of sample. Based on this approach, we found that specific bacteria transforming morphology into filament or elongated body strongly depends on cultivation temperature. Interestingly, we found that the morphologies of Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp. are elongated at below 25℃, above 20℃, above 15℃ and above 35℃, respectively. We envision the microfluidic device is a useful approach to analyze biological events with a high throughput manner.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (31)
참고문헌 신청
1. [학술지(정기간행물)] - Bereksi, N. / 2002 / Growth, morphology and surface properties of Listeria monocytogenes Scott A and LO28 under saline and acid environments / J. Appl. Microbiol 92 : 556 ~ 565
2. [학술지(정기간행물)] - Mattick, K. L. / 2000 / Habituation of Salmonella spp. at reduced water activity and its effect on heat tolerance / Appl. Environ. Micro-biol 66 : 4921 ~ 4925
3. [학술지(정기간행물)] - Zaika, L. L. / 2003 / Growth kinetics and cell morphology of Listeria monocytogenes Scott A as affected by temperature, NaCl, and EDTA / J. Food Prot 66 : 1208 ~ 1215
4. [학술지(정기간행물)] - Hazeleger, W. C. / 2006 / Fluorescence microscopy of NaCl-stressed, elongated Salmonella and Listeria cells reveals the presence of septa in filaments / Int. J. Food Microbiol 112 : 288 ~ 290
5. [학술지(정기간행물)] - McKenzie, R. L. / 2003 / Changes in biologically active ultraviolet radiation reaching the Earth's surface / Photochem. Photobiol. Sci 2 : 5 ~ 15
2. [학술지(정기간행물)] - Mattick, K. L. / 2000 / Habituation of Salmonella spp. at reduced water activity and its effect on heat tolerance / Appl. Environ. Micro-biol 66 : 4921 ~ 4925
3. [학술지(정기간행물)] - Zaika, L. L. / 2003 / Growth kinetics and cell morphology of Listeria monocytogenes Scott A as affected by temperature, NaCl, and EDTA / J. Food Prot 66 : 1208 ~ 1215
4. [학술지(정기간행물)] - Hazeleger, W. C. / 2006 / Fluorescence microscopy of NaCl-stressed, elongated Salmonella and Listeria cells reveals the presence of septa in filaments / Int. J. Food Microbiol 112 : 288 ~ 290
5. [학술지(정기간행물)] - McKenzie, R. L. / 2003 / Changes in biologically active ultraviolet radiation reaching the Earth's surface / Photochem. Photobiol. Sci 2 : 5 ~ 15
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UCI(KEPA) : I410-ECN-0101-2015-400-002657159