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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.6
- 수록면
- 155 - 164 (10page)
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초록· 키워드
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Succinic acid, a representative biomass-derived platform chemical, is a major fermentation product of Actinobacillus succinogenes. It is well known that carbon dioxide is consumed during the succinate fermentation, but the biochemical mechanism behind this phenomenon is not yet understood well. In this study, it was found that the addition of carbonic anhydrase (CA)s into media significantly enhances the succinic acid production by A. succinogenes during the fermentation supplied with carbon dioxide. It is likely that the (bi) carbonate produced by the CA activity from gaseous carbon dioxide is favoured by A. succinogenes for consumption and utilization. Therefore, the MgCO₃ requirement could be significantly reduced without compromising the succinate productivity. Furthermore, because of too high price of the commercial carbonic anhydrase, it was undertaken to economically overproduce a cyanobacterial carbonic anhydrase by the use of a recombinant Pichia pastoris. An expression vector system was constructed with the carbonic anhydrase gene PCR-cloned from Cyanobacterium Synechocystis sp., and introduced into P. pastoris for fermentation studies. About 95.9 g/L of succinic acid was produced in the production medium with 30 ppm of carbonic anhydrase, approximately 2 fold higher productivity compared to the parallel process with no supplementation of the enzyme. It is expected that this method can provide a valuable way of overcoming inefficiencies inherent in gas supply during CO₂-based bioprocesses like succinic acid fermentation.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (38)
참고문헌 신청
1. [학술지(정기간행물)] - Zeikus, J. G. / 1999 / Biotechnology of succinic acid production and markets for derived industrial products / APPl. Microbiol. Biotechnol 51 : 545 ~ 552
2. [학술지(정기간행물)] - McKinlay, J. B. / 2007 / Prospects for a bio-based succinate industry / Appl. Microbiol. Biotechnol 76 : 727 ~ 740
3. [학술지(정기간행물)] - Guettler, M. V. / 1999 / Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen / Int. J. Syst. Bacteriol 49 : 207 ~ 216
4. [학술지(정기간행물)] - Lee, P. C. / 2002 / Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E, from bovine rumen / Appl. Microbiol. Biotechnol 58 : 663 ~ 668
5. [학술지(정기간행물)] - Lin, H. / 2005 / Genetic reconstruction of the aerobic central metabolism in Escherichia coli for the absolute aerobic production of succinate / Biotechnol. Bioeng 89 : 148 ~ 156
2. [학술지(정기간행물)] - McKinlay, J. B. / 2007 / Prospects for a bio-based succinate industry / Appl. Microbiol. Biotechnol 76 : 727 ~ 740
3. [학술지(정기간행물)] - Guettler, M. V. / 1999 / Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen / Int. J. Syst. Bacteriol 49 : 207 ~ 216
4. [학술지(정기간행물)] - Lee, P. C. / 2002 / Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E, from bovine rumen / Appl. Microbiol. Biotechnol 58 : 663 ~ 668
5. [학술지(정기간행물)] - Lin, H. / 2005 / Genetic reconstruction of the aerobic central metabolism in Escherichia coli for the absolute aerobic production of succinate / Biotechnol. Bioeng 89 : 148 ~ 156
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