Purpose: This study investigates the factors affecting spirituality in nursing students. Method: A total of 247 nursing students participated in the study. Data were analyzed by frequencies, t-tests, ANOVA, Pearson correlation coefficients and multiple stepwise regression via SPSS WIN 18.0. Results: The mean scores for hope, gratitude, and spirituality were above-average. However, there were significant mean differences in spirituality according to religion, religious activity, voluntary service, satisfaction with the nursing field, and motivation for entering a college of nursing. Spirituality positively correlated with hope and gratitude. Significant predictors of spirituality included hope, gratitude, voluntary service and religious activity. The regression model explained 51.3% of perceived spirituality. Conclusion: To improve spirituality among nursing students, nursing educators should develop educational intervention programs to promote hope and gratitude, and support participation in voluntary service and religious activity.