지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 351 - 359 (9page)
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초록· 키워드
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For identifying the plasmid DNA coding cry gene of Bacillus thuringiensis subsp. aizawai KB098 with high insecticidal activity against Spodoptera exigua, mutant isolates with no crystal protein were produced by 42℃ incubation condition and then mutant plasmid DNA band patterns were compared with those of KB098. KB098 isolates had 4 cry genes, cry1Aa, cry1Ab, cry1C, cry1D, and also had been found seven plasmid DNA. Though the SDS-PAGE experiment, it was confirmed that mutant didn"t produce 130~145kDa protein band involved in bipyramidal shape crystal. Also, five mutant isolates had no cry genes coding plasmid DNA in PCR. In result of comparison the plasmid DNA of KB098 and 5 mutant isolates, only 1 plasmid DNA band was left out in mutant plasmid DNA pattern, so that the missing band was extracted from the gel. The missing(disappeared) plasmid DNA was the largest molecular size among the 7 plasmid DNA of KB098 and it was also confirmed this plasmid DNA had all 4 cry genes through PCR.
목차
Abstract
I. 서론
II. 재료 및 방법
III. 결과 및 고찰
참고문헌
참고문헌 (25)
참고문헌 신청
Abdullah MAF / 2009 / Manduca sexta(Lepidoptera : Sphingidae)cadherin fragments function as synergists for Cry1A and Cry1C Bacillus thuringiensis toxins against noctuid moths Helicoverpa zea, Agrotis ipsilon and Spodoptera exigua / Pest Management Science 65:1097~1103
Aronson AI / 1986 / Bacillus thuringiensis and related insect pathogens / Microbiological reviews 50:1~24
Bravo A / 2007 / Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control / Toxicon 49:423~435
Bravo A / 1998 / Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection / Applied and environmental microbiology 64:4965~4972
Chankhamhaengdecha ST / 2008 / Spore stage expression of a vegetative insecticidal gene increase toxicity of Bacillus thuringiensis subsp. aizawai SP41 against Spodoptera exigua / Journal of Biotechnology 136:122~112
Aronson AI / 1986 / Bacillus thuringiensis and related insect pathogens / Microbiological reviews 50:1~24
Bravo A / 2007 / Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control / Toxicon 49:423~435
Bravo A / 1998 / Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection / Applied and environmental microbiology 64:4965~4972
Chankhamhaengdecha ST / 2008 / Spore stage expression of a vegetative insecticidal gene increase toxicity of Bacillus thuringiensis subsp. aizawai SP41 against Spodoptera exigua / Journal of Biotechnology 136:122~112
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UCI(KEPA) : I410-ECN-0101-2016-480-000987234