지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Mouse hepatitis virus (MHV) is a major pathogen in laboratory mice that usually leads to fatal diseases, such as hepatitis, multiple sclerosis, encephalitis, and respiratory disease. MHV has a high infection rate, and it needs to be detected as soon as possible to prevent its spread to other facilities. However, MHV detection by enzyme-linked immunosorbent assay (ELISA) often gives false positives; thus, it is very important that the results are confirmed as true positives in the early infection stage or distinguished as false positives with more accurate, reliable methods. Under microbiological screening, MHV ELISA-positive mice were found in four GFP-tagging transgenic mice. To verify the detection of the MHV antigen directly, reverse transcription polymerase chain reaction (RT-PCR) was performed, and the mice were determined to be MHV negative. Additional serum antibody-based screening was conducted with three different ELISA kits, and multiplexed fluorometric immunoassay (MFIA) was performed to confirm their accuracy/sensitivity. In brief, the ELISA kit for A59 nucleocapsid protein (MHV-A59N) revealed MHV ELISA positivity, while other ELISA kits (MHV-S lysate and MHV-JHM lysate) demonstrated MHV negativity. In MFIA, only the test for the recombinant A59 nucleocapsid antigen was MHV positive, which was consistent with the ELISA results. These results suggest that the ELISA kit with the recombinant A59 nucleocapsid antigen might induce non-specific MHV ELISA positivity and that confirmation is therefore essential.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (13)
참고문헌 신청
1. [학술지(정기간행물)] - Barthold SW / 1993 / Enterotropic coronavirus (mouse hepatitis virus) in mice: influence of host age and strain on infection and disease / Lab Anim Sci 43 : 276 ~ 284
2. [학술지(정기간행물)] - Cajal N / 1962 / Experimental investigations of murine hepatitis. I. Evolution of hepatitis induced with MHV-2 andMHV-4 viruses in mice exposed to the action of radiophosphorus (P-32) and radioiodine (I-131) / Stud Cercet Inframicrobiol 13 : 163 ~ 167
3. [학술지(정기간행물)] - Eriksson KK / 2008 / Mouse hepatitis virus liver pathology is dependent on ADP-ribose-1''-phosphatase, a viral function conserved in the alpha-like supergroup / J Virol 82 : 12325 ~ 12334
4. [학술지(정기간행물)] - Guo JK / 2007 / The commonly used beta-actin-GFP transgenic mouse strain develops a distinct type of glomerulosclerosis / Transgenic Res 16 : 829 ~ 834
5. [학술지(정기간행물)] - Kagiyama N / 1990 / Studies on the development of an ELISA kit for microbiological monitoring. 2. Improvement of the prototype ELISA kit withspecial references to mouse hepatitis virus antigen / Jikken Dobutsu 39 : 97 ~ 102
2. [학술지(정기간행물)] - Cajal N / 1962 / Experimental investigations of murine hepatitis. I. Evolution of hepatitis induced with MHV-2 andMHV-4 viruses in mice exposed to the action of radiophosphorus (P-32) and radioiodine (I-131) / Stud Cercet Inframicrobiol 13 : 163 ~ 167
3. [학술지(정기간행물)] - Eriksson KK / 2008 / Mouse hepatitis virus liver pathology is dependent on ADP-ribose-1''-phosphatase, a viral function conserved in the alpha-like supergroup / J Virol 82 : 12325 ~ 12334
4. [학술지(정기간행물)] - Guo JK / 2007 / The commonly used beta-actin-GFP transgenic mouse strain develops a distinct type of glomerulosclerosis / Transgenic Res 16 : 829 ~ 834
5. [학술지(정기간행물)] - Kagiyama N / 1990 / Studies on the development of an ELISA kit for microbiological monitoring. 2. Improvement of the prototype ELISA kit withspecial references to mouse hepatitis virus antigen / Jikken Dobutsu 39 : 97 ~ 102
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UCI(KEPA) : I410-ECN-0101-2016-510-001347968