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자료유형
학술저널
저자정보
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제7권 제1호
발행연도
1977.6
수록면
55 - 62 (8page)

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A study was made to confirm both the validity and the applicablitity of influenza virus infectivity titration by the allatois-on-shell technique, originally devised by Far.ekas de St. Groth and White in 1958. Furthermore, a series of experiments was conducted to improve the infectivity titers of influenza virus type A, PR 8 strain by this method. The results are summarized as follows. Among the embryonated eggs tested, the 13-day old eggs were best suitable for successful deembryonation and for the preparation of allantois-on-shell pieces, and at the same time provided the highest infectivity titers. 2. Shaking incubation for 72 hours at 37'C resulted in higher infectivity titers than those of 48 hours of incubation. 3. Addition of mycostatin (20 units per ml) to Star.dard Medium effectively controlled the growth of fungi during the shaking incubation without any inhibitory effect on the virus titers. 4. Allocation of (virus) adsorption time (37C, 1 hr) prior to shakirg incubation of virus-inoculated allantois-on-shell pieces appeared to improve the results of infectivity titration. 5. Ambient temperature (room temperature or cold room) at which preparation of allantois-on-shell pieces, virus inoculation and setting up of the test were conducted did effect very little the infectivity titers when the (virus) adsorption period (37C, l hr) was applied before the shaking incubation. 6. A significant drop in the virus titers was observed when the infectivity titrations were carried out with sodiun bicarbonate-added Standard Medium. 7. Infectivity titers obtained by the allantois-on-shell technique were approximately 25 times lower than those of egg infectivity titrations.

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