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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제18권 제1호
발행연도
1988.6
수록면
25 - 42 (18page)

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As one of the attempts to select the adequate cell line to be used for propagation and maintenance of Human irnmunodeficiency virus(HIV) in laboratory, multiplication of the virus was followed up in human T leukemic cell, MT-2 and hurnan lymphoblastic leukemic cell, CCRF-CEM cells selected among various established cell lines. The results of this study were as follows : 1. In MT-2 cells, viral antigen began to be appeared at 1 days after inoculation of virus. Almost all cells were infected showing antigen-positive by immunofluorescence antibody (IFA) test 7 days after virus inoculation and the amounts of extracellualr viruses were maximal at 7 days postinfection. 2. In CCRF-CEM culture, viral antigen began to be appeared at 2 days after virus inoculation, but increased very slowly until 6 days postinfection. To show the maximal infection, it was required for 12 days and the amounts of extracellular viruses were maximal from 9 days after infection. 3. Cell associated viruses in MT-2 were maximal at 6 days postinfection and then decrea sed gradually. Therefore it was concluded that MT-2 is better cell than CCRF-CEM in the time needed to present the antigen in cells and in the amounts of virus produced.

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