Proteomic analysis of Helicobacter pylori J99 Outer Membrane Protein by Tandem Mass Spectrometry

Kyung-Mi Kim; Seung-Gyu Lee; Jung-Soo Joo; Young-Chul Kwon; Dong-Won Bea; Jea-Young Song; Hyung-Lyun Kang; Woo-Kon Lee; Myung-Je Cho; Kwang-Ho Rhee; Hee-Shang Youn; Seung-Chul Baik

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자료유형: 학술저널

저자정보: Kyung-Mi Kim (경상대학교) Seung-Gyu Lee (경상대학교) Jung-Soo Joo (경상대학교) Young-Chul Kwon (경상대학교) Dong-Won Bea (경상대학교) Jea-Young Song (경상대학교) Hyung-Lyun Kang (경상대학교) Woo-Kon Lee (경상대학교) Myung-Je Cho (경상대학교) Kwang-Ho Rhee (경상대학교) Hee-Shang Youn (경상대학교) Seung-Chul Baik (경상대학교)

저널정보: 대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY Journal of Bacteriology and Virology 제38권 제2호

발행연도: 2008.6

수록면: 53 - 60 (8page)

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The protein identity of sarcosine-insoluble outer membrane proteins (OMPs) of Helicobacter pylori J99 was determined with the basic study of understanding the function of proteins. A sarcosine-insoluble OMPs was resolved by two-dimensional electrophoresis with immobilized pH gradient strips. The most abundant proteins were shown in the alkaline pI regions (6.0~11.0) with molecular masses of 10 to 100 kDa. We have performed an extensive proteome analysis by quadrupole time of flight (Q-TOF) mass spectrometry (MS). Here, of 50 spots processed, 42 spots were identified, which represented 16 genes and we newly detected 8 kinds of proteins (JHP0119, JHP0388, JHP1046, JHP1405, JHP0073, JHP0551, JHP1382, JHP0552) from the sarcosin-insoluble fraction of H. pylori J99. Those may be used to elucidate the characterization of the OMPs of H. pylori J99, which will help identify new potential target proteins for vaccine development and drug therapy.

#Helicobacter pylori #Outer membrane protein #Q-TOF MS

참고문헌 (17)

참고문헌 신청

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