지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.6
- 수록면
- 103 - 108 (6page)
이용수
초록· 키워드
오류제보하기
Production of foreign proteins by transgenic plant cell cultures has several advantages such as post-translational modification, low risk of product contamination and low-cost production and purification. However, target proteins are degraded by extracellular proteases existing in the media. A solution to this problem is the use of perfusion culture and ion exchange chromatography for the application of integrated bioprocess using in situ recovery. With this method, production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in this study. First, optimization of cell concentration during the induction phase for the production of hGM-CSF was examined. As cell concentration increased, the level of hGM-CSF was decreased due to the presence of extracellular proteases. Induction using sugarfree media produced 33% more hGM-CSF. The effects of pH on the binding of hGM-CSF to cationic and anionic exchange resins were also investigated. In terms of stability, optimal pH was found to be 5~7. In the case of using buffer exchange when CM-Sepharose was used as a cationic exchange resin, optimal pH for binding was 4.8 and adsorption yield was 77%. When DEAE-Sepharose was used as an anionic exchange resin, it was 5.5 (74%). Without buffer exchange, optimal pH was 4.6 (84%). From these results, an integrated bioprocess using in situ recovery with simultaneous production and separation of foreign protein in transgenic plant cell suspension cultures was found to be feasible.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (19)
참고문헌 신청
[학술지(정기간행물)] - James, E. A. / 2000 / Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells / Protein Expr. Purif 19 : 131 ~ 138
[학술지(정기간행물)] - Zhang, X. W. / 1999 / Production of granulocyte macrophage colony stimulating factor (GM-CSF) by high cell density fermentation of secretory recombination Escherichia coli / Process Biochem 34 : 55 ~ 58
[학술지(정기간행물)] - Shin, Y. J. / 2003 / High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture / Biotechnol. Bioeng 82 : 778 ~ 783
[학술지(정기간행물)] - Sharma, A. K. / 2009 / Plants as bioreactors: Recent developments and emerging opportunities / Biotechnol. Adv 27 : 811 ~ 832
[학술지(정기간행물)] - Twyman, R. M. / 2003 / Molecular farming in plants: Host systems and expression technology / Trends Biotechnol 21 : 570 ~ 578
[학술지(정기간행물)] - Zhang, X. W. / 1999 / Production of granulocyte macrophage colony stimulating factor (GM-CSF) by high cell density fermentation of secretory recombination Escherichia coli / Process Biochem 34 : 55 ~ 58
[학술지(정기간행물)] - Shin, Y. J. / 2003 / High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture / Biotechnol. Bioeng 82 : 778 ~ 783
[학술지(정기간행물)] - Sharma, A. K. / 2009 / Plants as bioreactors: Recent developments and emerging opportunities / Biotechnol. Adv 27 : 811 ~ 832
[학술지(정기간행물)] - Twyman, R. M. / 2003 / Molecular farming in plants: Host systems and expression technology / Trends Biotechnol 21 : 570 ~ 578
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