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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.12
- 수록면
- 130 - 136 (7page)
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Human cytomegalovirus UL18, a MHC class I homologue, has been considered a natural killer (NK) cell decoy. It ligates LIR-1/ILT2 (CD85j), an NK inhibitory receptor, to prevent lysis of infected target cells. However, precise role of UL18 to NK cell cytotoxicity is yet elusive. Difficulty in clarifying the function of UL18 lies in complication in detecting UL18 mainly due to low level expression of UL18 on the surface and gradual loss of its expression. Methods: To overcome this hurdle, cDNA of cytoplasmic tail-less UL18 was constructed and expressed in swine endothelial cell (SEC). The expression level and its stability in the cell surface were monitored with FACS analysis. Results: Surface expression of UL18 is up-regulated by removing cytoplasmic tail portion from UL18F (a full sequence of UL18). SECs transfected with a cDNA of UL18CY (a cytoplasmic tail-less UL18) stably expressed UL18 molecule on the surface without gradual loss of its expression during 6 week continuous cultures. In the NK cytotoxicity assay, UL18 functions either inhibiting or activating NK cell cytotoxicity according to the source of NK cells. We found that there is individual susceptibility in determining whether the engagement of NK cell and UL18 results in overall inhibiting or activating NK cell cytotoxicity. Conclusion: In this study, we found that cytoplasmic tail is closely related to the regulatory function for controlling surface expression of UL18. Furthermore, by constructing stable cell line in which UL18 expression is up-regulated and stable, we provided a useful tool to clarify exact functions of UL18 on various immune cells having ILT2 receptor.
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참고문헌 (20)
참고문헌 신청
Wilkinson GW / 2008 / Modulation of natural killer cells by human cytomegalovirus / J Clin Virol 41:206~212
Fahnestock ML / 1995 / The MHC class I homolog encoded by human cytomegalovirus binds endogenous peptides / Immunity 3:583~590
Chapman TL / 1999 / The inhibitory receptor LIR-1 uses a common binding interaction to recognize class I MHC molecules and the viral homolog UL18 / Immunity 11:603~613
Leong CC / 1998 / Modulation of natural killer cell cytotoxicity in human cytomegalovirus infection: the role of endogenous class I major histocompatibility complex and a viral class I homolog / J Exp Med 187:1681~1687
Reyburn HT / 1997 / The class I MHC homologue of human cytomegalovirus inhibits attack by natural killer cells / Nature 386:514~517
Fahnestock ML / 1995 / The MHC class I homolog encoded by human cytomegalovirus binds endogenous peptides / Immunity 3:583~590
Chapman TL / 1999 / The inhibitory receptor LIR-1 uses a common binding interaction to recognize class I MHC molecules and the viral homolog UL18 / Immunity 11:603~613
Leong CC / 1998 / Modulation of natural killer cell cytotoxicity in human cytomegalovirus infection: the role of endogenous class I major histocompatibility complex and a viral class I homolog / J Exp Med 187:1681~1687
Reyburn HT / 1997 / The class I MHC homologue of human cytomegalovirus inhibits attack by natural killer cells / Nature 386:514~517
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