지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.12
- 수록면
- 264 - 274 (11page)
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초록· 키워드
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The unrestricted population of CD4<SUP>＋</SUP>Foxp3<SUP>＋</SUP> regulatory T (Treg) cells, which have been known to control the expression of autoimmune diseases and protective immunity to inflammatory reactions, has led to greater appreciation of functional plasticity. Detecting and/or isolating Ag-specific CD4<SUP>＋</SUP>Foxp3<SUP>＋</SUP> Tregs at the single cell level are required to study their function and plasticity. In this study, we established and compared both MHC class II tetramer and intracellular CD154 staining, in order to detect CD4<SUP>＋</SUP>Foxp3<SUP>＋</SUP> Treg specific for foreign Ag in acute and chronic infections with lymphocytic choriomeningitis virus (LCMV). Our results revealed that MHC class II tetramer staining showed a lower detection rate of LCMV GP<SUB>66-77</SUB>-specific CD4<SUP>＋</SUP> T cells because most of MHC class II tetramers were unbound and unstable when combined staining was performed with intracellular cytokines. In contrast, intracellular CD154 staining was revealed to be easier and simple for detecting LCMV GP<SUB>66-77</SUB>-specific CD4<SUP>＋</SUP> T cells, compared to MHC class II tetramer staining. Subsequently, we employed intracellular CD154 staining to detect LCMV GP<SUB>66-77</SUB>-specific CD4<SUP>＋</SUP>Foxp3<SUP>＋</SUP> Tregs using Foxp3<SUP>GFP</SUP> knock-in mouse, and found that LCMV GP<SUB>66-77</SUB>-specific CD4<SUP>＋</SUP>Foxp3<SUP>＋</SUP> Tregs and polyclonal CD4<SUP>＋</SUP>Foxp3<SUP>＋</SUP> Tregs showed differential expansion in mice infected with LCMV Arms or Cl13 at acute (8 and 13 days pi) and chronic phases (35 days pi). Therefore, our results provide insight into the valuable use of intracellular CD154 staining to detect and characterize foreign Ag-specific CD4<SUP>＋</SUP>Foxp3<SUP>＋</SUP> Treg in various models.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
References
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UCI(KEPA) : I410-ECN-0101-2016-511-001746008