지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 22 - 31 (10page)
이용수
초록· 키워드
오류제보하기
Objectives : Effects of nanoparticles including zinc oxide nanoparticles, titanium oxide nanoparticles, and their mixtures on skin corrosion and irritation were investigated by using in vitro 3D human skin models (KeraSkinTM) and the results were compared to those of an in vivo animal test.
Methods : Skin models were incubated with nanoparticles for a definite time period and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide method. Skin corrosion and irritation were identified by the decreased viability based on the pre-determined threshold.
Results : Cell viability after exposure to nanomaterial was not decreased to the pre-determined threshold level, which was 15% after 60 minutes exposure in corrosion test and 50% after 45 minutes exposure in the irritation test. IL-1α release and histopathological findings support the results of cell viability test. In vivo test using rabbits also showed non-corrosive and non-irritant results.
Conclusions : The findings provide the evidence that zinc oxide nanoparticles, titanium oxide nanoparticles and their mixture are ‘non corrosive’ and ‘non-irritant’ to the human skin by a globally harmonized classification system. In vivo test using animals can be replaced by an alternative in vitro test.
Methods : Skin models were incubated with nanoparticles for a definite time period and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide method. Skin corrosion and irritation were identified by the decreased viability based on the pre-determined threshold.
Results : Cell viability after exposure to nanomaterial was not decreased to the pre-determined threshold level, which was 15% after 60 minutes exposure in corrosion test and 50% after 45 minutes exposure in the irritation test. IL-1α release and histopathological findings support the results of cell viability test. In vivo test using rabbits also showed non-corrosive and non-irritant results.
Conclusions : The findings provide the evidence that zinc oxide nanoparticles, titanium oxide nanoparticles and their mixture are ‘non corrosive’ and ‘non-irritant’ to the human skin by a globally harmonized classification system. In vivo test using animals can be replaced by an alternative in vitro test.
목차
Introduction
Methods and Materials
Results
Discussion
References
참고문헌 (34)
참고문헌 신청
Ludi B / 2013 / Zinc oxide nanoparticles: chemical mech anisms and classical and non-classical crystallization / Dalton Trans 42(35):12554~12568
Ma H / 2013 / Ecotoxicity of manufactured ZnO nanoparticles--a review / Environ Pollut 172:76~85
Osmond MJ / 2010 / Zinc oxide nanoparticles in modern sunscreens:an analysis of potential exposure and hazard / Nanotoxicology 4(1):15~41
Gurr JR / 2005 / Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells / Toxicology 213(1):66~73
Sharma V / 2011 / Zinc oxide nanoparticle induced genotoxicity in primary human epidermal keratinocytes / J Nanosci Nanotechnol 11(5):3782~3788
Ma H / 2013 / Ecotoxicity of manufactured ZnO nanoparticles--a review / Environ Pollut 172:76~85
Osmond MJ / 2010 / Zinc oxide nanoparticles in modern sunscreens:an analysis of potential exposure and hazard / Nanotoxicology 4(1):15~41
Gurr JR / 2005 / Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells / Toxicology 213(1):66~73
Sharma V / 2011 / Zinc oxide nanoparticle induced genotoxicity in primary human epidermal keratinocytes / J Nanosci Nanotechnol 11(5):3782~3788
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UCI(KEPA) : I410-ECN-0101-2016-539-002083082