지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 319 - 327 (9page)
이용수
초록· 키워드
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Hemagglutination inhibition (HI) test employing whole virus antigen is a prescribed serological test for serotyping, diagnosis and surveillance for avian paramyxoviruses (APMVs). For use as alternative to the virus antigen, hemagglutininneuraminidase (HN) protein gene of the wild duck isolate APMV-6/WB12-163FS of APMV serotype 6 (APMV-6) was amplified, cloned and expressed in Spodoptera frugiperda insect cells. The HN gene of 1,842 bps in length showed nucleotide and amino acid homology of 93.4% and 97.1%, respectively with that of APMV-6 prototype strain. Putative sialic acid binding motif and potential N-linked glycosylation sites were conserved. In Western blot analysis, the expressed protein had a molecular mass of 66 kDa and reacted specifically with antiserum to APMV-6. In addition, the recombinant HN protein showed biological properties such as hemagglutination (HA) and elution. The recombinant HN protein produced from infected cells showed high HA titers (approximately 2<SUP>13</SUP> HA unit/ml). The HA activity of the recombinant HN protein was inhibited by antisera to APMV-6. In cross HA inhibition test, the recombinant HN protein had the highest titers with antisera to homologous APMV serotype, although there was weak cross reaction with some of antisera to other APMV serotypes. Our results indicated that recombinant APMV-6 HN protein would have the potential as alternative to the APMV-6 antigen in HI assays.
목차
INTRODUCTION
MATERIALS AND METHODS
RERSULTS
DISCUSSION
REFERENCES
참고문헌 (22)
참고문헌 신청
[단행본] - Alexander DJ / 1997 / Diseases of Poultry / Iowa state university press : 541 ~ 569
[학술지(정기간행물)] - Miller PJ / 2010 / Evidence for a new avian paramyxovirus serotype 10 detected in rockhopper penguins from the Falkland Islands / J Virol 84 : 11496 ~ 1504
[학술지(정기간행물)] - Briand FX / 2012 / Complete genome sequence of a novel avian paramyxovirus / J Virol 86 : 7710 ~
[학술지(정기간행물)] - Terregino C / 2013 / Antigenic and genetic analyses of isolate APMV/wigeon/Italy/3920-1/2005 indicate that it represents a new avian paramyxovirus (APMV-12) / Arch Virol 158 : 2233 ~ 2243
[학술지(정기간행물)] - Warke A / 2008 / Comparative study on the pathogenicity and immunogenicity of wild bird isolates of avian paramyxovirus 2, 4, and 6 in chickens / Avian Pathol 37 : 429 ~ 434
[학술지(정기간행물)] - Miller PJ / 2010 / Evidence for a new avian paramyxovirus serotype 10 detected in rockhopper penguins from the Falkland Islands / J Virol 84 : 11496 ~ 1504
[학술지(정기간행물)] - Briand FX / 2012 / Complete genome sequence of a novel avian paramyxovirus / J Virol 86 : 7710 ~
[학술지(정기간행물)] - Terregino C / 2013 / Antigenic and genetic analyses of isolate APMV/wigeon/Italy/3920-1/2005 indicate that it represents a new avian paramyxovirus (APMV-12) / Arch Virol 158 : 2233 ~ 2243
[학술지(정기간행물)] - Warke A / 2008 / Comparative study on the pathogenicity and immunogenicity of wild bird isolates of avian paramyxovirus 2, 4, and 6 in chickens / Avian Pathol 37 : 429 ~ 434
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UCI(KEPA) : I410-ECN-0101-2016-475-002249992