지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 233 - 240 (8page)
이용수
초록· 키워드
오류제보하기
Some virulence proteins of Helicobacter pylori, such as vacuolating cytotoxic protein A (VacA) and cytotoxinassociated gene protein A (CagA) have been reported to be causative agents of various gastric diseases including chronic gastritis, gastric ulcer or gastric adenocarcinoma. The expression level of these virulence proteins can be regulated when H. pylori is exposed to the antibacterial agent, cyanidin 3-O-glucoside (C3G) as previously reported. In this study, we analyzed the quantitative change of various virulence proteins including CagA and VacA by C3G treatment. We used 2-dimensional electrophoresis (2-DE) to analyze the quantitative change of representative ten proteome components of H. pylori 60190 (VacA<SUP>+</SUP>/CagA<SUP>+</SUP>; standard strain of Eastern type). After 2-DE analysis, spot intensities were analyzed using ImageMaster<SUP>TM</SUP> 2-DE Platinum software then each spot was identified using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) or peptide sequencing using Finnigan LCQ ion trap mass spectrometer (LC-MS/MS). Next, we selected major virulence proteins of H. pylori among quantitatively meaningful ten spots and confirmed the 2-DE results by Western blot analysis. These results suggest that cyanidin 3-O-glucoside can modulate a variety of H. pylori pathogenic determinants.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (28)
참고문헌 신청
[학술지(정기간행물)] - Alm RA / 1999 / Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori / Nature 14 : 176 ~ 180
[학술지(정기간행물)] - Backert S / 2005 / Subproteomes of soluble and structure-bound Helicobacter pylori proteins analyzed by two-dimensional gel electrophoresis and mass spectrometry / Proteomics 5 : 1331 ~ 1345
[학술지(정기간행물)] - Censini S / 1996 / cag pathogenicity island of Helicobacter pylori encodes type I-specific and disease-associated virulence factors / Proc Natl Acad Sci U S A 93 : 14648 ~ 14653
[학술지(정기간행물)] - Cho MJ / 2002 / Identifying the major proteome components of Helicobacter pylori strain 26695 / Electrophoresis 23 : 1161 ~ 1173
[학술지(정기간행물)] - Cover TL / 1993 / Correlation between vacuolating cytotoxin production by Helicobacter pylori isolates in vitro and in vivo / Infect Immun 61 : 5008 ~ 5012
[학술지(정기간행물)] - Backert S / 2005 / Subproteomes of soluble and structure-bound Helicobacter pylori proteins analyzed by two-dimensional gel electrophoresis and mass spectrometry / Proteomics 5 : 1331 ~ 1345
[학술지(정기간행물)] - Censini S / 1996 / cag pathogenicity island of Helicobacter pylori encodes type I-specific and disease-associated virulence factors / Proc Natl Acad Sci U S A 93 : 14648 ~ 14653
[학술지(정기간행물)] - Cho MJ / 2002 / Identifying the major proteome components of Helicobacter pylori strain 26695 / Electrophoresis 23 : 1161 ~ 1173
[학술지(정기간행물)] - Cover TL / 1993 / Correlation between vacuolating cytotoxin production by Helicobacter pylori isolates in vitro and in vivo / Infect Immun 61 : 5008 ~ 5012
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UCI(KEPA) : I410-ECN-0101-2016-510-002322036