지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 307 - 312 (6page)
이용수
초록· 키워드
오류제보하기
Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and kLa. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (23)
참고문헌 신청
[학술지(정기간행물)] - Fisher, R. / 2000 / Molecular farming of pharmaceutical proteins / Transgenic Res 9 : 279 ~ 299
[학술지(정기간행물)] - Hellwig, S. / 2004 / Plant cell cultures for the production of recombinant proteins / Nature Biotechnol 22 : 1415 ~ 1422
[학술지(정기간행물)] - James, E. / 2006 / Loss and recovery of protein productivity in genetically modified plant cell lines / Plant Cell Rep 25 : 723 ~ 727
[학술지(정기간행물)] - Pree, I. / 2006 / New approaches to prevent transplant rejection: Co-stimulation blockers anti-CD40L and CTLA4Ig / Drug Discov. Today 3 : 41 ~ 47
[학술지(정기간행물)] - Lee, S.-J. / 2007 / Production and characterization of human CTLA4Ig expressed in transgenic rice cell suspension cultures / Protein Exp. Purif 51 : 293 ~ 302
[학술지(정기간행물)] - Hellwig, S. / 2004 / Plant cell cultures for the production of recombinant proteins / Nature Biotechnol 22 : 1415 ~ 1422
[학술지(정기간행물)] - James, E. / 2006 / Loss and recovery of protein productivity in genetically modified plant cell lines / Plant Cell Rep 25 : 723 ~ 727
[학술지(정기간행물)] - Pree, I. / 2006 / New approaches to prevent transplant rejection: Co-stimulation blockers anti-CD40L and CTLA4Ig / Drug Discov. Today 3 : 41 ~ 47
[학술지(정기간행물)] - Lee, S.-J. / 2007 / Production and characterization of human CTLA4Ig expressed in transgenic rice cell suspension cultures / Protein Exp. Purif 51 : 293 ~ 302
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UCI(KEPA) : I410-ECN-0101-2016-470-002231751