지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: This study was performed to investigate the anti-tumor effect of O. japonicus extracts on intrahepatic cholangiocarcinoma cell line SNU-1079.
Methods: Cholangiocarcinoma SNU-1079 cells were treated with various concentrations of O. japonicus DW and EtOH extracts (0-300 ㎍/㎖) for 24, 48 or 72 h. Cell viability was evaluated through a PMS/MTS assay, and the apoptosis rate was examined through ELISA assay and flow cytometry analysis. The mRNA expression of apoptosisand cell cycle progression-related genes (Bcl-2, Mcl-1, Bax, Survivin, Cyclin D1, and p21) was evaluated using real-time PCR, and the caspase activity was examined using immunoblot analysis.
Results: O. japonicus extracts inhibited cell proliferation and increased apoptosis rate in both ELISA assay and flow cytometry analysis. O. japonicus extracts decreased Bcl-2, Mcl-1, Survivin, and Cyclin D1 mRNA expression and increased Bax mRNA level. O. japonicus extracts also increased Caspase-3 activation. Overall, O. japonicus DW extracts were more effective than EtOH extracts.
Conclusions: O. japonicus inhibited cell proliferation and induced apoptosis in SNU-1079 cells via mitochondria-mediated intrinsic pathway, which leads to Caspase-3 activation. The results indicate that O. japonicus is a potential therapeutic herb with anti-tumor effect against intrahepatic cholangiocarcinoma.
Methods: Cholangiocarcinoma SNU-1079 cells were treated with various concentrations of O. japonicus DW and EtOH extracts (0-300 ㎍/㎖) for 24, 48 or 72 h. Cell viability was evaluated through a PMS/MTS assay, and the apoptosis rate was examined through ELISA assay and flow cytometry analysis. The mRNA expression of apoptosisand cell cycle progression-related genes (Bcl-2, Mcl-1, Bax, Survivin, Cyclin D1, and p21) was evaluated using real-time PCR, and the caspase activity was examined using immunoblot analysis.
Results: O. japonicus extracts inhibited cell proliferation and increased apoptosis rate in both ELISA assay and flow cytometry analysis. O. japonicus extracts decreased Bcl-2, Mcl-1, Survivin, and Cyclin D1 mRNA expression and increased Bax mRNA level. O. japonicus extracts also increased Caspase-3 activation. Overall, O. japonicus DW extracts were more effective than EtOH extracts.
Conclusions: O. japonicus inhibited cell proliferation and induced apoptosis in SNU-1079 cells via mitochondria-mediated intrinsic pathway, which leads to Caspase-3 activation. The results indicate that O. japonicus is a potential therapeutic herb with anti-tumor effect against intrahepatic cholangiocarcinoma.
목차
Introduction
Materials and Methods
Results
Discussion and Conclusion
References
참고문헌 (51)
참고문헌 신청
[학술지(정기간행물)] - Rizvi S / 2013 / Pathogenesis, diagnosis, and management of cholangiocarcinoma / Gastroenterology 145 : 1215 ~ 1229
[학술지(정기간행물)] - Shaib Y / 2004 / The epidemiology of cholangiocarcinoma / Seminars in liver disease (24) : 115 ~ 125
[학술지(정기간행물)] - Welzel TM / 2006 / Impact of classification of hilar cholangiocarcinomas(Klatskin tumors)on the incidence of intra-and extrahepatic cholangiocarcinoma in the United States / Journal of the National Cancer Institute 98 : 873 ~ 875
[학술지(정기간행물)] - Fava G / 2012 / Molecular pathogenesis of cholangiocarcinoma / Int J Hepatol 2012 : 630543 ~
[학술지(정기간행물)] - Lazaridis KN / 2005 / Cholangiocarcinoma / Gastroenterology 128 : 1655 ~ 1667
[학술지(정기간행물)] - Shaib Y / 2004 / The epidemiology of cholangiocarcinoma / Seminars in liver disease (24) : 115 ~ 125
[학술지(정기간행물)] - Welzel TM / 2006 / Impact of classification of hilar cholangiocarcinomas(Klatskin tumors)on the incidence of intra-and extrahepatic cholangiocarcinoma in the United States / Journal of the National Cancer Institute 98 : 873 ~ 875
[학술지(정기간행물)] - Fava G / 2012 / Molecular pathogenesis of cholangiocarcinoma / Int J Hepatol 2012 : 630543 ~
[학술지(정기간행물)] - Lazaridis KN / 2005 / Cholangiocarcinoma / Gastroenterology 128 : 1655 ~ 1667
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UCI(KEPA) : I410-ECN-0101-2016-519-002648778