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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.3
- 수록면
- 125 - 132 (8page)
이용수
초록· 키워드
오류제보하기
High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a beadcapture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency (Et) in a highly significant manner.
목차
1. Introduction
2. Theory
3. Materials and Methods
4. Results
5. Discussion
References
참고문헌 (26)
참고문헌 신청
A. Øren, C. Husebø, A.-C. Iversen, and R. Austgulen, J. Immunol. Meth. 303, 1 (2005).
J. Oberteuffer, IEEE Trans. Magn. 9, 303 (1973).
F. Paul, S. Roath, and D. Melville, Br. J. Haematol. 38, 273 (1978).
F. Paul, S. Roath, D. Melville, D. Warhurst, and J. Osisanya, The Lancet 318, 70 (1981).
S. Miltenyi, W. Müller, W. Weichel, and A. Radbruch, Cytometry 11, 231 (1990).
J. Oberteuffer, IEEE Trans. Magn. 9, 303 (1973).
F. Paul, S. Roath, and D. Melville, Br. J. Haematol. 38, 273 (1978).
F. Paul, S. Roath, D. Melville, D. Warhurst, and J. Osisanya, The Lancet 318, 70 (1981).
S. Miltenyi, W. Müller, W. Weichel, and A. Radbruch, Cytometry 11, 231 (1990).
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UCI(KEPA) : I410-ECN-0101-2016-428-002656203