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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.3
- 수록면
- 27 - 32 (6page)
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초록· 키워드
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Several CoA transferases from Clostridium beijerinckii, C. perfringens and Klebsiella pneumoniae were examined for biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) in recombinant Escherichia coli XL1-Blue strain. The CB3819 gene and the CB4543 gene from C. beijerinckii, the pct gene from C. perfringens and the pct gene from K. pneumoniae, which encodes putative CoA transferase gene, respectively, was co-expressed with the Pseudomonas sp. MBEL 6-19 phaC1437 gene encoding engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 (PhaC1Ps6-19) to examine its activity for the construction of key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli XL1-Blue expressing the phaC1437 gene and CB3819 gene synthesized poly(3-hydroxybutyrate) [P(3HB)] homopolymer to the P(3HB) content of 60.5 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 3-hydroxybutyrate. Expression of the phaC1437 gene and CB4543 gene in recombinant E. coli XL1-Blue also produced P(3HB) homopolymer to the P(3HB) content of 51.2 wt% in the same culture condition. Expression of the phaC1437 gene and the K. pneumoniae pct gene in recombinant E. coli XL1-Blue could not result in the production of PHAs in the same culture condition. However, the recombinant E. coli XL1-Blue expressing the phaC1437 gene and the C. perfringens gene could produce poly(3-hydroxybutyrate-co-lactate [P(86.4mol%3HB-co- 13.7 mol%LA) up to the PHA content of 10.6 wt% in the same culture condition. Newly examined CoA transfereases in this study may be useful for the construction of engineered E. coli strains to produce PHA containing novel monomer such lactate.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (13)
참고문헌 신청
Oh, Y. H., I. Y. Eom, J. C. Joo, J. H. Yu, B. K. Song, S. H. Lee, S. H. Hong, and S. J. Park (2015) Recent advances in development of biomass pretreatment technologies used in biorefinery for the production of bio-based fuels, chemicals and polymers. Korean J. Chem. Eng. 32: 1945-1959.
Lee, S. Y. (1996) Bacterial polyhydroxyalkanoates. Biotechnol. Bioeng. 49: 1-14.
Steinbüchel, A. and H. E. Valentin (1995) Diversity of bacterial polyhydroxyalkanoic acid. FEMS Microbiol. Lett. 128: 219-228.
Jung, Y. K., T. Y. Kim, S. J. Park, and S. Y. Lee (2010) Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers. Biotechnol. Bioeng. 105: 161-171.
Park, S. J., S. Y. Lee, T. W. Kim, Y. K. Jung, and T. H. Yang (2012) Biosynthesis of lactate containing polyesters by metabolically engineered bacteria. Biotechnol. J. 7:199-212.
Lee, S. Y. (1996) Bacterial polyhydroxyalkanoates. Biotechnol. Bioeng. 49: 1-14.
Steinbüchel, A. and H. E. Valentin (1995) Diversity of bacterial polyhydroxyalkanoic acid. FEMS Microbiol. Lett. 128: 219-228.
Jung, Y. K., T. Y. Kim, S. J. Park, and S. Y. Lee (2010) Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers. Biotechnol. Bioeng. 105: 161-171.
Park, S. J., S. Y. Lee, T. W. Kim, Y. K. Jung, and T. H. Yang (2012) Biosynthesis of lactate containing polyesters by metabolically engineered bacteria. Biotechnol. J. 7:199-212.
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