Effect of Methylene Blue-mediated Photodynamic Therapy on Wild-type and Ciprofloxacin-resistant Mycobacterium smegmatis

Insoo Shim; Myungwon Choi; Yegee Min; Kwang Hyuk Seok; Jiyeun Kate Kim; Jee-Yeong Jeong; Chul-Ho Oak; Indal Park

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자료유형: 학술저널

저자정보: Insoo Shim (Kosin University College of Kosin University College of Medicine) Myungwon Choi (Kosin University College of Medicine) Yegee Min (Kosin University College of Medicine) Kwang Hyuk Seok (Kosin University College of Medicine) Jiyeun Kate Kim (Kosin University College of Medicine) Jee-Yeong Jeong (Kosin University College of Medicine) Chul-Ho Oak (Kosin University College of Medicine) Indal Park (Kosin University College of Medicine)

저널정보: 대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY JOURNAL OF BACTERIOLOGY AND VIROLOGY Vol.46 No.1

발행연도: 2016.3

수록면: 27 - 35 (9page)

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Tuberculosis (TB) patients are normally treated with a combination of antibiotics. However, with improper or incomplete treatment of antibiotics, the disease may progress to multidrug-resistant TB (MDR-TB). The treatment of MDR-TB is very costly and inefficient. Therefore, there is a great demand of new therapeutic approaches for MDR-TB such as photodynamic therapy. In this study, we tried to optimize the conditions for photodynamic inactivation of TB using methylene blue as a photosensitizer. Different combinations of methylene blue concentrations and light doses were tested for their photodynamic effects to A549 cells or Mycobacterium smegmatis (M. smegmatis). We also tested the effect of photodynamic therapy on ciprofloxacin-resistant M. smegmatis. Methylene blue treatment alone did not affect the survival rates of A549 cells or bacteria up to 5 μg/ml. When the A549 and M. smegmatis cells treated with methylene blue were irradiated with laser light (wavelength, 630 nm), photodynamic inactivation of cells was increased in methylene blue concentration- and light dose-dependent manners. Interestingly, the ciprofloxacin-resistant M. smegmatis exhibited higher level of susceptibility to methylene blue-mediated photodynamic inactivation. This study suggests that photodynamic therapy at 3.6 J/cm² in the presence of 5 μg/ml methylene blue may be an appropriate range for therapy due to the high bactericidal activity against high level of ciprofloxacin-resistant M. smegmatis and the low damaging effect to mammalian cells. This study demonstrates that photodynamic therapy could be a potential alternative for MDR-TB treatment.

#Photodynamic therapy #Methylene blue #Mycobacterium smegmatis #Ciprofloxacin #Tuberculosis

참고문헌 (27)

참고문헌 신청

[학술지(정기간행물)] - Dougherty TJ / 1993 / Photodynamic therapy / Photochem Photobiol 58 : 895 ~ 900

[학술지(정기간행물)] - Allison RR / 2004 / Photosensitizers in clinical PDT / Photodiag Photodynamic 1 : 27 ~ 42

[학술지(정기간행물)] - Brancaleon L / 2002 / Laser and non-laser light sources for photodynamic therapy / Lasers Med Sci 17 : 173 ~ 186

[학술지(정기간행물)] - Daniela V / 2015 / Bacterial photodynamic inactivation mediated by methylene blue and red light is enhanced by synergistic effect of potassium iodide / Antimicrob Agents Chemother 59 : 5203 ~ 5212

[학술지(정기간행물)] - Dolmans DE / 2003 / Photodynamic therapy for cancer / Nat Rev Cancer 3 : 380 ~ 387

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Insoo Shim

소속기관 Kosin University College of Kosin University College of Medicine

주요연구분야 의약학 > 기초의학 > 미생물학 자연과학 > 생물학

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