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논문 기본 정보

자료유형
학술저널
저자정보
Jae-Yeong Han (Chungnam National University) Chan-Hwan Park (Chungnam National University) Eun-Yeong Seo (Chungnam National University) Jung-Kyu Kim (Chungnam National University) John Hammond (United States National Arboretum, Floral and Nursery Plants Research Unit) Hyoun-Sub Lim (Chungnam National University)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.43 No.1
발행연도
2016.3
수록면
21 - 27 (7page)

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Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) have been known to induce top working disease causing economical damage in apple. Occurrences of these three viruses in pome fruit trees, including apple, have been reported around the world. The transmission of the three viruses was reported by grafting, and there was no report of transmission through mechanical contact, insect vector, or seed except some herbaceous hosts of ASGV. As RNA extraction methods for fruit trees, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and multiplex RT-PCR techniques have been improved for reliability and stability, and low titer viruses that could not be detected in the past have become detectable. We studied the seed transmission ability of three apple viruses through apple seedling diagnosis using RT-PCR. Nineteen seeds obtained from commercially grown apple were germinated and two of the resulting plants were ASGV positive. Seven clones of the amplified ASGV coat protein (CP) genes of these isolates were sequenced. Overall sequence identities were 99.84% (nucleotide) and 99.76% (amino acid). Presence of a previously unreported single nucleotide and amino acid variation conserved in all of these clones suggests a possible association with seed transmission of these ‘S’ isolates. A phylogenetic tree constructed using ASGV CP nucleotide sequences showed that isolate S sequences were grouped with Korean, Chinese, Indian isolates from apple and Indian isolates from kiwi.
#apple stem grooving virus #seed transmission #RT-PCR

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
References

참고문헌 (17)

참고문헌 신청
Allen WR. 1969. Occurrence and seed transmission of Tomato bushy stunt virus in apple. Canadian Journal ofPlant Science 49:797-799. google schola Campbell AI. 1963. The effect of some latent virus infections on the growth and cropping of apples. Journal ofHorticultural Science 38:15-19. google schola Hadidi A, Hansen AJ, Parish CL, Yang X. 1991. Scar skin and dapple apple viroids are seed-borne andpersistent in infected apple trees. Research in Virology 142(4):289-296. google schola Han JY, Kim JK, Cheong JS, Seo EY, Park CH, Ju HK, Cho IS, Gotoh T., Moon JS, Hammond J, Lim HS.2015. Survey of Apple chlorotic leaf spot virus and Apple stem grooving virus occurrence in Korea andfrequency of mixed infections in Apple. Journal of the Faculty of Agriculture, Kyushu University.60(2):323-329. google schola Inouye N, Maeda T, Mitsuhata K. 1979. Citrus tatter leaf virus isolated from lily. Annals of the PhytopathologicalSociety of Japan 45(5):712-720. google schola

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UCI(KEPA) : I410-ECN-0101-2016-480-002738426