지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.6
- 수록면
- 9 - 18 (10page)
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초록· 키워드
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Purpose: Wnt signaling plays an essential role in the dental epithelium and mesenchyme during tooth morphogenesis. Deletion of the Wntless (Wls) gene in odontoblasts appears to reduce canonical Wnt activity, leading to inhibition of odontoblast maturation. However, it remains unclear if autonomous Wnt ligands are necessary for differentiation of dental pulp cells into odontoblast-like cells to induce reparative dentinogenesis, one of well-known feature of pulp repair to form tertiary dentin.
Materials and Methods: To analyze the autonomous role of Wls for differentiation of dental pulp cells into odontoblast-like cells, we used primary dental pulp cells from unerupted molars of Wls-floxed allele mouse after infection with adenovirus for Cre recombinase expression to knockout the floxed Wls gene or control GFP expression. The differentiation of dental pulp cells into odontoblast-like cells was analyzed by quantitative real-time polymerase chain reaction.
Result: Proliferation rate was significantly decreased in dental pulp cells with Cre expression for Wls knockout. The expression levels of Osterix (Osx), runt-related transcription factor 2 (Runx2), and nuclear factor I-C (Nfic) were all significantly decreased by 0.3-fold, 0.2-fold, and 0.3-fold respectively in dental pulp cells with Wls knockout. In addition, the expression levels of Bsp, Col1a1, Opn, and Alpl were significantly decreased by 0.7-fold, 0.3-fold, 0.8-fold, and 0.6-fold respectively in dental pulp cells with Wls knockout.
Conclusion: Wnt ligands produced autonomously are necessary for proper proliferation and odontoblastic differentiation of mouse dental pulp cells toward further tertiary dentinogenesis.
Materials and Methods: To analyze the autonomous role of Wls for differentiation of dental pulp cells into odontoblast-like cells, we used primary dental pulp cells from unerupted molars of Wls-floxed allele mouse after infection with adenovirus for Cre recombinase expression to knockout the floxed Wls gene or control GFP expression. The differentiation of dental pulp cells into odontoblast-like cells was analyzed by quantitative real-time polymerase chain reaction.
Result: Proliferation rate was significantly decreased in dental pulp cells with Cre expression for Wls knockout. The expression levels of Osterix (Osx), runt-related transcription factor 2 (Runx2), and nuclear factor I-C (Nfic) were all significantly decreased by 0.3-fold, 0.2-fold, and 0.3-fold respectively in dental pulp cells with Wls knockout. In addition, the expression levels of Bsp, Col1a1, Opn, and Alpl were significantly decreased by 0.7-fold, 0.3-fold, 0.8-fold, and 0.6-fold respectively in dental pulp cells with Wls knockout.
Conclusion: Wnt ligands produced autonomously are necessary for proper proliferation and odontoblastic differentiation of mouse dental pulp cells toward further tertiary dentinogenesis.
목차
Introduction
Materials and Methods
Result
Discussion
Conclusion
References
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - Goldberg M / 2004 / Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering / Crit Rev Oral Biol Med 15 : 13 ~ 27
[학술지(정기간행물)] - Mitsiadis TA / 2004 / Parallels between tooth development and repair: conserved molecular mechanisms following carious and dental injury / J Dent Res 83 : 896 ~ 902
[학술지(정기간행물)] - Balic A / 2010 / Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars / Bone 46 : 1639 ~ 1651
[학술지(정기간행물)] - Sagomonyants K / 2014 / Biphasic effects of FGF2on odontoblast differentiation involve changes in the BMP and Wnt signaling pathways / Connect Tissue Res 55 (Suppl 1) : 53 ~ 56
[학술지(정기간행물)] - Ricucci D / 2014 / Is hard tissue formation in the dental pulp after the death of the primary odontoblasts a regenerative or a reparative process? / J Dent 42 : 1156 ~ 1170
[학술지(정기간행물)] - Mitsiadis TA / 2004 / Parallels between tooth development and repair: conserved molecular mechanisms following carious and dental injury / J Dent Res 83 : 896 ~ 902
[학술지(정기간행물)] - Balic A / 2010 / Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars / Bone 46 : 1639 ~ 1651
[학술지(정기간행물)] - Sagomonyants K / 2014 / Biphasic effects of FGF2on odontoblast differentiation involve changes in the BMP and Wnt signaling pathways / Connect Tissue Res 55 (Suppl 1) : 53 ~ 56
[학술지(정기간행물)] - Ricucci D / 2014 / Is hard tissue formation in the dental pulp after the death of the primary odontoblasts a regenerative or a reparative process? / J Dent 42 : 1156 ~ 1170
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UCI(KEPA) : I410-ECN-0101-2017-515-000850390