지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.6
- 수록면
- 105 - 112 (8page)
이용수
초록· 키워드
오류제보하기
Ferritin is known to regulate iron metabolism and maintain iron in a variety of the eukaryotic organisms. The region encoding the mature ferritin (0.47 kb, H-type) of Periserrula leucophryna was amplified using the designed primers including restriction enzyme site and termination codon and subcloned in frame to the pRBAS secretion vector containing the signal sequence, RBS, and promoter of amylase gene (E. coli-Bacillus shuttle vector), resulting in recombinant pRBASPLF vector. Recombinant ferritin (18 kDa) was correctly processed and secreted from Bacillus subtilis LKS strain harboring the pRBAS-PLF vector and quantitatively analyzed by SDS-PAGE and western blot, respectively. Secretion of the ferritin was optimized by culture conditions (host, medium, temperature, nitrogen source) in 3 L batch culture and 5 L jar fermenter. Finally. the ferritin was largely produced using 50 L fermenter as the following conditions; at 30℃, 150 rpm, 1 vvm in Bacillus subtilis LKS using PY medium. The secreted ferritin was maximally measured (approximately 177.6 ug/ml) when the cell density reached to 14.4 at OD<SUB>600</SUB> (20 h incubation). The iron binding activity was confirmed by Perls" staining in 7.5% non-denaturing gel, indicating that the multimeric ferritin (composed of 24 subunits) was formed in the culture broth after secretion. Biologically, the culture broth and powder type containing ferritin were tested for possibility as feed additive in chicken broiler. As a result, the ferritin stimulated the growth of chick broil and improved feed efficiency and production index.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESUTLS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (31)
참고문헌 신청
[학술지(정기간행물)] - Andrew, S. C. / 1992 / Structure, function, and evolution of ferritins / J. Inorg. Biochem 47 : 161 ~ 174
[학술지(정기간행물)] - Theil, E. C. / 1987 / Ferritin: structure, gene, regulation, and cellular function in animals, plants, and microorganisms / Ann. Rev. Biochem 56 : 289 ~ 315
[학술지(정기간행물)] - Harrison, P. M. / 1996 / The ferritins: molecular properties, iron storage function and cellular regulation / Biochim. Biophys. Acta 1275 : 161 ~ 203
[학술지(정기간행물)] - Baynes, R. D. / 1990 / Iron deficiency / Ann. Rev. Nutr 10 : 133 ~ 148
[학술지(정기간행물)] - Theil, E. C. / 1990 / Regulation of ferritin and transferrin receptor mRNAs / J. Biol. Chem 265 : 4771 ~ 4774
[학술지(정기간행물)] - Theil, E. C. / 1987 / Ferritin: structure, gene, regulation, and cellular function in animals, plants, and microorganisms / Ann. Rev. Biochem 56 : 289 ~ 315
[학술지(정기간행물)] - Harrison, P. M. / 1996 / The ferritins: molecular properties, iron storage function and cellular regulation / Biochim. Biophys. Acta 1275 : 161 ~ 203
[학술지(정기간행물)] - Baynes, R. D. / 1990 / Iron deficiency / Ann. Rev. Nutr 10 : 133 ~ 148
[학술지(정기간행물)] - Theil, E. C. / 1990 / Regulation of ferritin and transferrin receptor mRNAs / J. Biol. Chem 265 : 4771 ~ 4774
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UCI(KEPA) : I410-ECN-0101-2017-470-000801979