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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.12
- 수록면
- 250 - 254 (5page)
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초록· 키워드
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Contamination of microcystins, a family of heptapeptide hepatotoxins, in eutrophic water bodies is a worldwide problem. Due to their poisoning effects on animals and humans, there is a requirement to characterize and quantify all microcystins present in a sample. As microcystins are, for most part, intracellular toxins produced by some genera of cyanobacteria, lysing cyanobacterial cells to release all microcystins is considered an important step. To date, although many cell lysis methods have been used, little work has been conducted comparing the results of those different methods. In this study, various methods for cell lysis and toxin extraction from the cell lysates were investigated, including sonication, bead beating, freeze/thaw, lyophilization and lysing with TritonX-100 surfactant. It was found that lyophilization, followed by extraction with 75% methanol, was the most effective for extracting toxins from Microcystis aeruginosa cells. Another important step prior to the analysis is removing impurities and concentrating the target analyte. For these purposes, a C18 Sep-Pak solid phase extraction cartridge was used, with the percentage of the eluent methanol also evaluated. As a result, methanol percentages higher than 75% appeared to be the best eluting solvent in terms of microcystin-leucine-arginine recovery efficiency for the further chromatographic and mass spectrometric analyses.
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등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
1. [학술지(정기간행물)] - Sangolkar, L. N / 2006 / Methods 111for determining microcystins (peptide hepatotoxins) and microcystin-producing cyanobacteria / Water Res 40 (19) : 3485 ~ 3496
2. [단행본] - World Health Organization / 2008 / Guidelines for drinking-water quality: incorporating 1st and 2nd addenda / WHO
3. [학술지(정기간행물)] - McElhiney, J / 2005 / Detection of the 333cyanobacterial hepatotoxins microcystins / Toxicol. Appl. Pharmacol 203 (3) : 219 ~ 230
4. [학술지(정기간행물)] - Yuan, M / 2006 / Microcystin 444analysis in human sera and liver from human fatalities in Caruaru, Brazil 1996 / Toxicon 48 (6) : 627 ~ 640
5. [학술지(정기간행물)] - Dai, M / 2008 / Simultaneous 555determination of microcystin-LR and its glutathione conjugate in fish tissues by liquid chromatography-tandem mass spectrometry / J. Chromatogr. B Analyt. Technol. Biomed. Life Sci 862 (1-2) : 43 ~ 50
2. [단행본] - World Health Organization / 2008 / Guidelines for drinking-water quality: incorporating 1st and 2nd addenda / WHO
3. [학술지(정기간행물)] - McElhiney, J / 2005 / Detection of the 333cyanobacterial hepatotoxins microcystins / Toxicol. Appl. Pharmacol 203 (3) : 219 ~ 230
4. [학술지(정기간행물)] - Yuan, M / 2006 / Microcystin 444analysis in human sera and liver from human fatalities in Caruaru, Brazil 1996 / Toxicon 48 (6) : 627 ~ 640
5. [학술지(정기간행물)] - Dai, M / 2008 / Simultaneous 555determination of microcystin-LR and its glutathione conjugate in fish tissues by liquid chromatography-tandem mass spectrometry / J. Chromatogr. B Analyt. Technol. Biomed. Life Sci 862 (1-2) : 43 ~ 50
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UCI(KEPA) : I410-ECN-0101-2017-539-001547370