지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.3
- 수록면
- 35 - 39 (5page)
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초록· 키워드
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In this study, multiparametric flow cytometry (FCM) was installed to enumerate the diagnosis of Pseudomonas aeruginosa ATCC 10145 and Escherichia coli K12 (IFO 3301). The nucleic acids (DNA/RNA) were double stained by a LIVE/DEAD bacLight viability kit, involving green SYTO 9 and red propidium iodide (PI), based on the permeability of two chemicals according to the integrity of plasma membrane. As the results showed, the gate for dead bacteria was defined as the range of 0.2 × 100 to 6.0 × 101 photo multiplier tube (PMT) 2 fluorescence (X-axis) and 2.0 × 100 to 2.0 × 102 PMT 4 fluorescence (Y-axis), and the gate for live bacteria was defined as the range of 6.0 × 100 to 6.0 × 102 PMT 2 fluorescence (X-axis) and 2.0 × 100 to 4.0 × 102 PMT 4 fluorescence (Y-axis). In the comparison of the number of the tested bacteria detected by FCM (viability assessment) and plate culture (cultivability assessment), the number of bacteria detected by FCM well represented the number of bacteria that was detected by the colony forming unit (CFU) counting method when bacteria were exposed to isopropyl alcohol and silver/copper cations. Consequently, it is concluded that the application of FCM to monitor the functional effect of disinfectants on the physiological status of target bacteria can offer more rapid and reliable data than the plate culture colony counting method.
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참고문헌 (22)
참고문헌 신청
1. [학술지(정기간행물)] - Akin EW / 1982 / Waterborne outbreak control: which disinfectant? / Environ. Health. Perspect 46 : 7 ~ 12
2. [학술지(정기간행물)] - Hwang MG / 2006 / Effect of intracellular resuscitation of Legionella pneumophila in Acanthamoeba polyphage cells on the antimicrobial properties of silver and copper / Environ. Sci. Technol 40 : 7434 ~ 7439
3. [학술지(정기간행물)] - Lebaron P / 1998 / Effectiveness of SYTOX green stain for bacterial viability assessment / Appl. Environ. Microbiol 64 : 2697 ~ 2700
4. [학술지(정기간행물)] - Barer MR / 1999 / Bacterial viability and culturability / Adv. Microb. Physiol 41 : 93 ~ 137
5. [학술지(정기간행물)] - Amor KB / 2002 / Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress / Appl. Environ. Microbiol 68 : 5209 ~ 5216
2. [학술지(정기간행물)] - Hwang MG / 2006 / Effect of intracellular resuscitation of Legionella pneumophila in Acanthamoeba polyphage cells on the antimicrobial properties of silver and copper / Environ. Sci. Technol 40 : 7434 ~ 7439
3. [학술지(정기간행물)] - Lebaron P / 1998 / Effectiveness of SYTOX green stain for bacterial viability assessment / Appl. Environ. Microbiol 64 : 2697 ~ 2700
4. [학술지(정기간행물)] - Barer MR / 1999 / Bacterial viability and culturability / Adv. Microb. Physiol 41 : 93 ~ 137
5. [학술지(정기간행물)] - Amor KB / 2002 / Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress / Appl. Environ. Microbiol 68 : 5209 ~ 5216
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UCI(KEPA) : I410-ECN-0101-2017-539-001548099