지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
시드로포어인 2, 3-dihydroxybenzoic acid (DHB)를 생산하는 Acinetobacter sp. B-W의 플라스미드를 분석한 결과, 20 kb 크기의 플라스미드를 함유하였다. 배양 온도 43°C가 플라스미드가 제거된 돌연변이체 생산에 효과적이었다. 이 돌연변이체는 2,3-DHB 생산 능력을 소실하였으며, chrome azurol S(CAS) 아가 배지에서 시드로포어 생산이 검출되지 않았다. 포도당과 황산 망간을 함유한 배지에서 28°C로 3일간 배양한 B-W 원 균주와 돌연변이체의 배양 상등액의 pH는 각각 4.5와 8.5로 나타났다. 돌연변이체에서는 ampicillin, actinomycin D, bacitracin, lincomycin과 vancomycin 같은 항생제에 대한 저항성이 사라졌으며, 이러한 항생제에 대한 최소 억제 농도(MIC)가 급격하게 감소하였다. B-W 균주에서 분리한 플라스미드로 대장균을 형질전환시킨 결과, 원 균주와 같은 크기의 플라스미드가 이 형질전환 대장균에서 발견되었다. 플라스미드가 제거된 돌연변이체에서는 플라스미드가 발견되지 않았다. 20 kb 크기의 플라스미드에 2,3-DHB 생산 유전자와 여러 항생제 저항성 유전자가 자리잡고 있는 것으로 추정된다.
목차
ABSTRACT
Materials and Methods
Results and Discussion
적요
References
참고문헌 (21)
참고문헌 신청
[학술지(정기간행물)] - Arnow, L. E / 1937 / Colorimetric determination of the components of 3, 4-dihydroxyphenylalanine-tyrosine mixtures / J. Biol. Chem 18 : 531 ~ 537
[학술지(정기간행물)] - Bauer, A. W. / 1966 / Antibiotic susceptibility testing by a standardized single disk method / Am. J. Clin. Pathol 45 : 493 ~ 496
[학술지(정기간행물)] - Chen, Q. / 1994 / Chromosome-mediated 2, 3-dihydroxybenzoic acid is a precursor in the biosynthesis of the plasmid mediated siderophore anguibactin in Vibrio anguillarum / J. Bacteriol 176 : 4226 ~ 4234
[기타자료] - Clinical and Laboratory Standards Institute / 2010 / Performance Standards for Antimicrobial Susceptibility Testing: Twentieth Informational Supplement M100-S20 / CLSI
[학술지(정기간행물)] - Driss, F. / 2011 / Relationship between plasmid loss and gene expression in Bacillus thuringiensis / Curr. Microbiol 62 : 1287 ~ 1293
[학술지(정기간행물)] - Bauer, A. W. / 1966 / Antibiotic susceptibility testing by a standardized single disk method / Am. J. Clin. Pathol 45 : 493 ~ 496
[학술지(정기간행물)] - Chen, Q. / 1994 / Chromosome-mediated 2, 3-dihydroxybenzoic acid is a precursor in the biosynthesis of the plasmid mediated siderophore anguibactin in Vibrio anguillarum / J. Bacteriol 176 : 4226 ~ 4234
[기타자료] - Clinical and Laboratory Standards Institute / 2010 / Performance Standards for Antimicrobial Susceptibility Testing: Twentieth Informational Supplement M100-S20 / CLSI
[학술지(정기간행물)] - Driss, F. / 2011 / Relationship between plasmid loss and gene expression in Bacillus thuringiensis / Curr. Microbiol 62 : 1287 ~ 1293
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UCI(KEPA) : I410-ECN-0101-2017-475-001371019