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논문 기본 정보

자료유형
학술저널
저자정보
봉기문 (전남생물산업진흥원) 김종민 (전남생물산업진흥원) 유재홍 (국립농업과학원) 박인철 (국립농업과학원) 이철원 (전남대학교) 김평일 (전남생물산업진흥원)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.31 No.3 (Wn.158)
발행연도
2016.9
수록면
158 - 164 (7page)

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Plant growth promoting (PGP) hormones, which are produced in a small quantity by bacteria, affect in plant growth and development. PGPs play an important role on the crop productivity in agricultural field. In this study, a photosynthetic bacterial strain producing the PGP was isolated from paddy soil. Bacterial isolate was gram negative, rod-shaped and motility positive. From the 16s rRNA gene sequence analysis, the isolate was identified as Rhodobacter capsulatus PS-2. The mass cultivation of R. capsulatus PS-2 was optimized by considering of the carbon, nitrogen and inorganic salt sources. Optimal medium composition was determined as Na-succinate 4.5 g, yeast extract 5 g, K<SUB>2</SUB>HPO<SUB>4</SUB> 1 g, MgSO<SUB>4</SUB> 5 g , per liter. From the result of 500 L fermentation for 2 days using the optimal medium, the viable cells were 8.7×109 cfu/mL. R. capsulatus PS-2 strain produced the carotenoid and indole-3-acetic acid (IAA). The carotenoid extraction and quantitative analysis were performed by HCl-assisting method. Total carotenoid contents from R. capsulatus PS-2 culture broth were measured as 7.02±0.04 and 6.93±0.05 mg/L under photoheterotrophic and chemoheterotrophic conditions, respectively. To measure the productivity of IAA, colorimetric method was employed using Salkowski reagent at optical density 535 nm. The results showed that the highest content of IAA was 197.44±5.92 mg/L in the optimal medium supplemented with 0.3% tryptophan.

목차

Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
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UCI(KEPA) : I410-ECN-0101-2017-470-001334588