지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 263 - 269 (7page)
이용수
초록· 키워드
오류제보하기
For decades, the microorganisms in arctic soils have been newly discovered according to the climate change and global warming. In this study, the chemical structure of a lipid A molecule from Pseudomonas sp. strain PAMC 28615 which was newly discovered from arctic soils was characterized by mass spectrometric approaches such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI multi-stage tandem mass spectrometry (MS). First, lipopolysaccharide (LPS) from Pseudomonas sp. strain PAMC 28615 was extracted and subsequently hydrolyzed to obtain the lipid A. The parent ion peak at m/z 1632 was determined by MALDI-TOF MS, which also can validate our lipid A purification method. For detailed structural determination, we performed the multiple-stage tandem mass analysis (MS<SUP>4</SUP>) of the parent ion, and subsequently the abundant fragment ions in each MS stage are tested. The fragment ions in each MS stage were produced from the loss of phosphate groups and fatty acyl groups, which could be used to confirm the composition or the position of the lipid A components. Consequently, the mass spectrometry-based lipid A profiling method could provide the detail chemical structure of lipid A from the Pseudomonas sp. strain PAMC 28615 as an arctic bacterium from the frozen arctic soil.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - Romanovsky, V. / 2010 / Thermal state of permafrost in Russia / Permafr. Periglac. Process 21 : 136 ~ 155
[학술지(정기간행물)] - Brown, J. / 2008 / Report from the International Permafrost Association: State of permafrost in the first decade of the 21st century / Permafr. Periglac. Process 19 : 255 ~ 260
[학술지(정기간행물)] - Soina, V. S. / 2004 / The structure of resting bacterial populations in soil and subsoil permafrost / Astrobiology 4 : 345 ~ 358
[학술지(정기간행물)] - Crevecoeur, S. / 2015 / Bacterial community structure across environmental gradients in permafrost thaw ponds: methanotroph-rich ecosystems / Front. Microbiol 6 : 192 ~
[학술지(정기간행물)] - Glauser, M. / 1991 / Septic shock: pathogenesis / Septic shock: pathogenesis 338 : 732 ~ 736
[학술지(정기간행물)] - Brown, J. / 2008 / Report from the International Permafrost Association: State of permafrost in the first decade of the 21st century / Permafr. Periglac. Process 19 : 255 ~ 260
[학술지(정기간행물)] - Soina, V. S. / 2004 / The structure of resting bacterial populations in soil and subsoil permafrost / Astrobiology 4 : 345 ~ 358
[학술지(정기간행물)] - Crevecoeur, S. / 2015 / Bacterial community structure across environmental gradients in permafrost thaw ponds: methanotroph-rich ecosystems / Front. Microbiol 6 : 192 ~
[학술지(정기간행물)] - Glauser, M. / 1991 / Septic shock: pathogenesis / Septic shock: pathogenesis 338 : 732 ~ 736
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UCI(KEPA) : I410-ECN-0101-2017-470-001981612