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Comparison of chemiluminescence immunoassay (CIA) and immunochromatography assay(ICA) for Detecting HBsAg and Anti-HBs
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B형 간염항원 및 항체 검사를 위한 화학발광면역검사법(CIA)과 면역크로마토그래피법(ICA)의 비교분석

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Academic journal
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Journal
Korea Academy Industrial Cooperation Society Journal of the Korea Academia-Industrial cooperation Society Vol.14 No.7 KCI Accredited Journals
Published
2013.7
Pages
3,419 - 3,424 (6page)

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Comparison of chemiluminescence immunoassay (CIA) and immunochromatography assay(ICA) for Detecting HBsAg and Anti-HBs
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Diverse immunoassays including a chemiluminescent immunoassay (CIA) are used to detect hepatitis B surface antigen (HBsAg) and antibody (anti-HBs). Recently, an increasing number of institutions have been utilizing an immunochromatography assay (ICA), which is easy to use. In this study, We evaluated ICA kits for the rapid detection of HBsAg and anti-HBs by comparing them with a CIA. A total of 120 serum hospital samples, were collected for the whole month, were assayed using ICA kit. The Concordance rate, sensitivity, specificity, positive predictive value and negative predictive value of the ICA for HBsAg based on CIA results were 97%, 97%, 100%, 100%, and 96.8%, respectively. The diagnostic performances of the ICA for Anti-HBs were 90%, 90%, 93.3%, 93.1%, and 90.3%, respectively. The ICA kit failed to detect HBsAg and anti-HBs in low reactive samples. The ICA kits for the rapid detection of HBsAg might be recommended for interpreted with caution and dual analysis in the clinical laboratory.
#Chemiluminescent immunoassay #HBsAg #Anti-HBs #Immunochromatography assay

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References (16)

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1. [학술지(정기간행물)] - Y. F. Liaw / 2009 / Hepatitis B virus infection / Lancet 14 : 582 ~ 592 Crossref 2. [학술지(정기간행물)] - S. Y. Kwon / 2011 / Epidemiology and prevention of hepatitis B virus infection / Korean J Hepatol 17 : 87 ~ 95 google schola 3. [학술지(정기간행물)] - O. D. Oh / 1997 / Taxol of radioimmunoassay for I-125 label compounds / Korean J Biomed Lab 3 : 125 ~ 130 google schola 4. [학술지(정기간행물)] - S. Yagci / 2012 / Comparison of Monolisa HCV Ag/Ab ULTRA With Two Anti-HCV Assays For the Detection of HCV Infection in Hospital Setting / Curr Microbiol 64 : 148 ~ 151 google schola 5. [학술지(정기간행물)] - 송선미 / 2002 / 자동 면역분석기 ARCHITECT i2000을 이용한 B형 간염 혈청학적 표지자 검사 평가 / Annals of Laboratory Medicine 22 (1) : 42 ~ 46 dbpia

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