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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.93 No.4
- 발행연도
- 2017.10
- 수록면
- 173 - 180 (8page)
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초록· 키워드
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Purpose: In order to suggest optimal anticancer drugs for patient-tailored chemotherapy, we developed a colorectal cancer (CRC)-liver metastasis patient-derived tumor xenograft (PDTX) model.
Methods: Tissue obtained from a patient with CRC-liver metastasis (F0) was transplanted in a nonobese female mouse with diabetic/severe combined immune deficiency (F1) and the tumor tissue was retransplanted into nude mice (F2). When tumor volumes reached ~500 mm3, the F2 mice were randomly divided into 4 groups (n = 4/group) of doxorubicin, cisplatin, docetaxel, and nontreated control groups. The tumor tissues were investigated using H&E staining, terminal deoxynucleotidyl transferase dUTP nick end labeling assays, and immunohistochemistry. To determine where the mutant allele frequencies varied across the different passages, we isolated genomic DNA from the primary tumor, liver metastasis, and PDTX models (F1/F2).
Results: The physiological properties of the tumor were in accord with those of the patient’s tumors. Anticancer drugs delayed tumor growth, inhibited proliferation, and caused apoptosis. Histological assessments revealed no observable heterogeneity among the intragenerational PDTX models. Target exon sequencing analysis without high-quality filter conditions revealed some genetic variations in the 83 cancer-related genes across the generations. However, when de novo mutations were defined as a total count of zero in F0 and ≥5 in F2, exactly prognostic impact of clone cancer profiling (EGFR, KRAS, BRAF, PIK3CA, NRAS, APC and TP53) were detected in the paired.
Conclusion: A CRC liver metastasis PDTX model was established for the evaluation of chemotherapeutic efficacy. This model retained the physiological characters of the patient tumors and potentially provides a powerful means of assessing chemotherapeutic efficacy.
Methods: Tissue obtained from a patient with CRC-liver metastasis (F0) was transplanted in a nonobese female mouse with diabetic/severe combined immune deficiency (F1) and the tumor tissue was retransplanted into nude mice (F2). When tumor volumes reached ~500 mm3, the F2 mice were randomly divided into 4 groups (n = 4/group) of doxorubicin, cisplatin, docetaxel, and nontreated control groups. The tumor tissues were investigated using H&E staining, terminal deoxynucleotidyl transferase dUTP nick end labeling assays, and immunohistochemistry. To determine where the mutant allele frequencies varied across the different passages, we isolated genomic DNA from the primary tumor, liver metastasis, and PDTX models (F1/F2).
Results: The physiological properties of the tumor were in accord with those of the patient’s tumors. Anticancer drugs delayed tumor growth, inhibited proliferation, and caused apoptosis. Histological assessments revealed no observable heterogeneity among the intragenerational PDTX models. Target exon sequencing analysis without high-quality filter conditions revealed some genetic variations in the 83 cancer-related genes across the generations. However, when de novo mutations were defined as a total count of zero in F0 and ≥5 in F2, exactly prognostic impact of clone cancer profiling (EGFR, KRAS, BRAF, PIK3CA, NRAS, APC and TP53) were detected in the paired.
Conclusion: A CRC liver metastasis PDTX model was established for the evaluation of chemotherapeutic efficacy. This model retained the physiological characters of the patient tumors and potentially provides a powerful means of assessing chemotherapeutic efficacy.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (20)
참고문헌 신청
[학술지(정기간행물)] - 정규원 / 2015 / Cancer Statistics in Korea: Incidence, Mortality, Survival, and Prevalence in 2012 / Cancer Research and Treatment 47 (2) : 127 ~ 141
[학술지(정기간행물)] - Puig I / 2013 / A personalized preclinical model to evaluate the metastatic potential of patient-derived colon cancer initiating cells / Clin Cancer Res 19 : 6787 ~ 6801
[학술지(정기간행물)] - Bendell JC / 2016 / A Phase II Study of FOLFOXIRI Plus Panitumumab Followed by Evaluation for Resection in Patients With Metastatic KRAS Wild-Type Colorectal Cancer With Liver Metastases Only / Oncologist 21 : 279 ~ 280
[학술지(정기간행물)] - Akgul O / 2014 / Role of surgery in colorectal cancer liver metastases / World J Gastroenterol 20 : 6113 ~ 6122
[학술지(정기간행물)] - Whittle JR / 2015 / Patient-derived xenograft models of breast cancer and their predictive power / Breast Cancer Res 17 : 17 ~
[학술지(정기간행물)] - Puig I / 2013 / A personalized preclinical model to evaluate the metastatic potential of patient-derived colon cancer initiating cells / Clin Cancer Res 19 : 6787 ~ 6801
[학술지(정기간행물)] - Bendell JC / 2016 / A Phase II Study of FOLFOXIRI Plus Panitumumab Followed by Evaluation for Resection in Patients With Metastatic KRAS Wild-Type Colorectal Cancer With Liver Metastases Only / Oncologist 21 : 279 ~ 280
[학술지(정기간행물)] - Akgul O / 2014 / Role of surgery in colorectal cancer liver metastases / World J Gastroenterol 20 : 6113 ~ 6122
[학술지(정기간행물)] - Whittle JR / 2015 / Patient-derived xenograft models of breast cancer and their predictive power / Breast Cancer Res 17 : 17 ~
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UCI(KEPA) : I410-ECN-0101-2018-514-001328125