지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Transforming growth factor beta (TGF-β) signaling pathways are involved in the regulation of proliferation, differentiation, immunity, survival, and apoptosis of many cells. The aim of this study was to investigate the differential expression of TGF-β-related genes, and their interactions and regulators in the spleen of two genetically disparate chicken lines (Marek’s disease resistant line 6.3 and Marek’s disease-susceptible line 7.2) induced with necrotic enteritis (NE) by Eimeria maxima and Clostridium perfringens infection. By using high-throughput RNA-sequencing, we investigated 76 TGF-β-related genes that were significantly and differentially expressed in the spleens of the chickens. Approximately 20 TGF-β pathway genes were further verified by qRT-PCR, and the results were consistent with our RNA sequencing data. All 76 identified genes were analyzed through Gene Ontology and mapped onto the KEGG chicken TGF-β pathway. Our results demonstrated that several key genes, including TGF-β1-3, bone morphogenetic proteins (BMP)1-7, inhibitor of differentiation (ID) proteins ID1-3, SMAD1-9, and Jun, showed a markedly differential expression between the two chicken lines, relative to their respective controls. We then further predicted 24 known miRNAs that targeted BMP7 mRNA from 139 known miRNAs in the two chicken lines. Among these, six miRNAs were measured by qRT-PCR. In conclusion, this study is the first to analyze most of the genes, interactions, and regulators of the TGF-β pathway in the innate immune responses of NE afflicted chickens.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONCLUSION
REFERENCES
참고문헌 (35)
참고문헌 신청
[학술지(정기간행물)] - Braun J / 2010 / Downregulation of micro RNAs directs the EMT and invasive potential of anaplastic thyroid carcinomas / Oncogene 29 (29) : 4237 ~ 4244
[학술지(정기간행물)] - Burks TN / 2011 / Role of TGF-beta signaling in inherited and acquired myopathies / Skelet Muscle 1 (1) : 19 ~
[학술지(정기간행물)] - de Jonge RR / 1997 / Frequent inactivation of the transforming growth factor beta type II receptor in small-cell lung carcinoma cells / Oncol Res 9 (2) : 89 ~ 98
[학술지(정기간행물)] - Dinh H / 2014 / Modulation of micro-RNAs in two genetically disparate chicken lines showing different necrotic enteritis disease susceptibility / Vet Immunol Immunopathol 159 (1-2) : 74 ~ 82
[학술지(정기간행물)] - Escalona R / 2012 / Transforming growth factor beta mRNA and protein expression in the ovary of the chicken embryo / Growth Factors 30 (5) : 297 ~ 303
[학술지(정기간행물)] - Burks TN / 2011 / Role of TGF-beta signaling in inherited and acquired myopathies / Skelet Muscle 1 (1) : 19 ~
[학술지(정기간행물)] - de Jonge RR / 1997 / Frequent inactivation of the transforming growth factor beta type II receptor in small-cell lung carcinoma cells / Oncol Res 9 (2) : 89 ~ 98
[학술지(정기간행물)] - Dinh H / 2014 / Modulation of micro-RNAs in two genetically disparate chicken lines showing different necrotic enteritis disease susceptibility / Vet Immunol Immunopathol 159 (1-2) : 74 ~ 82
[학술지(정기간행물)] - Escalona R / 2012 / Transforming growth factor beta mRNA and protein expression in the ovary of the chicken embryo / Growth Factors 30 (5) : 297 ~ 303
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Analysis of MAPK Signaling Pathway Genes in the Intestinal Mucosal Layer of Necrotic Eenteritis-Afflicted Two Inbred Chicken Lines
한국가금학회지
2017 .09
-
miR-200a-3p regulates immune response in necrotic enteritis-afflicted chickens
한국가금학회 정기총회 및 학술발표회
2022 .11
-
Identification of differentially expressed genes in the intestinal mucosa of two inbred lines afflicted with Necrotic Enteritis
한국가금학회 정기총회 및 학술발표회
2015 .11
-
Effects of Lycopene on the Expression of Metabolic and Inflammatory Related Genes in Chickens
한국가금학회 정기총회 및 학술발표회
2015 .11
-
닭의 품종에 따른 텔로미어 함량과 면역 관련 유전자의 발현양상
한국가금학회 정기총회 및 학술발표회
2022 .11
-
Identification of Female Specific Genes in the W Chromosome that are Expressed during Gonadal Differentiation in the Chicken
한국가금학회지
2019 .12
-
Pre-determined cell fate by asymmetric expression of signaling pathway-related genes during intrauterine development in chicken
한국가금학회 정기총회 및 학술발표회
2017 .11
-
Genomic Regions associated with Necrotic Enteritis Resistance in Fayoumi and White Leghorn Chickens
한국가금학회지
2015 .03
-
The potential of non-movement behavior observation method for detection of sick broiler chickens
한국축산학회지
2023 .03
-
Functional analysis of miRNA-146b on chicken myoblast cells
한국가금학회 정기총회 및 학술발표회
2019 .06
-
Analysis of Consumers' Preferences and Price Sensitivity to Native Chickens
한국축산식품학회지
2017 .01
-
한국의 토종닭 유전자원의 보존 현황 조사 연구
Journal of Animal Breeding and Genomics
2022 .12
-
Differences in liver microRNA profiling in pigs with low and high feed efficiency
한국축산학회지
2022 .03
-
Mitogen-activated protein kinase pathway modulates immune response of chicken macrophages via chicken avian β-defensin 8
한국가금학회 정기총회 및 학술발표회
2022 .11
-
Identification of the chicken type by comparison of signature of selection between Korean native chicken and commercial layer
한국가금학회 정기총회 및 학술발표회
2019 .06
-
Metagenomic analysis on chicken from farm to market using 16S rRNA gene targeted sequencing
한국식품영양과학회 학술대회발표집
2022 .10
-
Biology and Potential Use of Chicken Bone Marrow-derived Cells
한국수정란이식학회지
2018 .01
-
한국토종닭의 육종 선발 지표 탐색
한국가금학회 정기총회 및 학술발표회
2024 .11
-
Identification of the chicken type by comparison of signature of selection between Korean native chicken and commercial broiler
한국가금학회 정기총회 및 학술발표회
2019 .06
-
Humulone promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast
한국식품영양과학회 학술대회발표집
2022 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-527-001355632