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논문 기본 정보

자료유형
학술저널
저자정보
Yu Wang (Dalian Polytechnic University) Kang-Duk Choi (Hankyong National University) Hongshan Yu (Dalian Polytechnic University) Fengxie Jin (Dalian Polytechnic University) Wan-Taek Im (Hankyong National University)
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.40 No.2
발행연도
2016.4
수록면
121 - 126 (6page)

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초록· 키워드

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Background: Ginsenoside F1, a pharmaceutical component of ginseng, is known to have antiaging, antioxidant, anticancer, and keratinocyte protective effects. However, the usage of ginsenoside F1 is restricted owing to the small amount found in Korean ginseng.
Methods: To enhance the production of ginsenoside F1 as a 10 g unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the commercial enzyme Cellulase KN from Aspergillus niger with food grade, which has ginsenoside-transforming ability. The proposed optimum reaction conditions of Cellulase KN were pH 5.0 and 50℃.
Results: Cellulase KN could effectively transform the ginsenosides Re and Rg1 into F1. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 5.0 and 50℃ for 48 h with protopanaxatriol-type ginsenoside mixture (at a concentration of 10 mg/mL) from ginseng roots. Finally, 13.0 g of F1 was produced from 50 g of protopanaxatriol-type ginsenoside mixture with 91.5±1.1% chromatographic purity.
Conclusion: The results suggest that this enzymatic method could be exploited usefully for the preparation of ginsenoside F1 to be used in cosmetic, functional food, and pharmaceutical industries.

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ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusion
References

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