지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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In this study, we set up an analytical method that can be used for rapid and accurate determination of representative isoquinoline alkaloids in medicinal plants using UPLC-ESI-Q-TOF MS (ultra pressure liquid chromatography–electrospray ionization–quadrupole–time-of-flight mass spectrometry). The compounds were eluted on a C18 column with 0.1 % formic acid and acetonitrile, and separated with good resolution within 13 min. Each of the separated components was characterized by precursor ions (generated by ESI-Q-TOF) and fragment ions (produced by collision-induced dissociation, CID), which were used as a reliable database. We also performed method validation: analytes showed excellent linearity (R2, 0.9971–0.9996), LOD (5–25 ng/mL), LOQ (17–82 ng/mL), accuracy (91.6–97.4 %) as well as intra- and inter-day precisions (RSD, 1.8–3.2 %). In the analysis of Chelidonium majus L., magnoflorine, coptisine, sanguinarine, berberine and palmatine were detected by matching retention times and characteristic fragment ion patterns of reference standards. We also confirmed that, among the quantified components, coptisine was present in the highest quantity. Furthermore, alkaloid profiling was carried out by analyzing the fragment ion patterns corresponding to peaks of unknown components. In this manner, protopine, chelidonine, stylopine, dihydroberberine, canadine, and nitidine were tentatively identified. We also proposed the molecular structure of the fragment ions that appear in the mass spectrum. Therefore, we concluded that our suggested method for the determination of major isoquinoline alkaloids by UPLC-Q-TOF can be useful not only for quality control, but also for rapid and accurate investigation of phytochemical constituents of medicinal plants.
목차
Abstract
1. Introduction
2. Experiments
3. Result and Discussion
4. Conclusions
References
참고문헌 (32)
참고문헌 신청
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Z. Zhang, B. Li, X. Meng, S. Yao, L. Jin, J. Yang, J. Wang, H. Zhang, Z. Zhang, D. Cai, Y. Zhang and G. Ning, Sci. Rep., 6, 20848 (2016).
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UCI(KEPA) : I410-ECN-0101-2018-433-001587760