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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 130 - 140 (11page)
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초록· 키워드
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In the bio-synthesis of poly(3-hydroxybutyrate)(PHB), which is a kind of poly(3-hydroxyalkanoate)(PHA), aimed to control the low molecular weight of PHB and obtain a telechelic PHB. As a result of incubation of R. eutropha at 30℃ with ethylene glycol added as a chain transfer agent, PHB content on the dry cell weight increased up to 24h, however, it decreased after that, and the molecular weight of PHB increased from 9h to 12h, and then, decreased up to 72h. The decrease of the content and the molecular weight of PHB indicates that PHB was decomposed as an energy source in bacterial cells and was incorporated into metabolic pathways. 1H-NMR of the obtained PHB after incubation for 72h was measured to determine the terminal groups of the PHB during incubation. As the results of １H-NMR measurement, the peaks derived from ethylene glycol in both terminals of PHB were observed. Which indicate that the terminal reaction was caused by the addition of ethylene glycol, and that telechelic PHB having hydroxyl group at the both terminals where molecular weight was controlled was successfully synthesized.
목차
Abstract
1. 서론
2. 실험
3. 결과 및 고찰
4. 결론
References
참고문헌 (24)
참고문헌 신청
G. Q. Chen, AMicrobial Polyhydroxyalkanoates (PHA) Based Bio- and Materials Industry, Chem. Soc. Rev., 38(8), 2434(2009).
S. Y. Dai and Z. Li, Enzymatic Preparation of Novel ThermoplasticDi-blockCopolyestersContainingPoly[(R) -3-hydroxybutyrate] and Poly(epsilon-caprolactone) Blocks viaRing-opening Polymerization, Biomacromolecules, 9(7), 1883(2008).
S. Fiedler, A. Steinb?chel, and B. H. A. Rehm, PhaGmediated Synthesis of Poly(3-hydroxyalkanoates) Consisting of Medium-chain-length Constituents from NonrelatedCarbonSources inRecombinantPseudomonas Fagi, Appl. Environ.Microbiol., 66(5), 2117(2000).
C. S. Ha and W. J. Cho, Miscibility, Properties, and Biodegradability of Microbial Polyester Containing Blends, Prog. Polym. Sci., 27(4), 759(2002).
S. K. Misra, S. P. Valappil, I. Roy, andA. R. Boccaccini, Polyhydroxyalkanoate(PHA)/inorganic PhaseComposites for Tissue EngineeringApplications, Biomacromolecules, 7(8), 2249(2006).
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UCI(KEPA) : I410-ECN-0101-2018-577-003122251