지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 83 - 98 (16page)
- DOI
- 10.17519/apiculture.2018.06.33.2.83
이용수
초록· 키워드
오류제보하기
Sacbrood virus (SBV) is one of the main honeybee pathogens. It has been detected worldwide. Five genotypes of SBV were proposed based on the analysis of coding DNA sequences (CDSs) and deduced amino acids. However, a reliable tool to examine each SBV-genotype has not been developed yet. To establish a PCR system for SBV-genotyping, designation of specific primer-pairs and construction of standard DNAs for 5 genotypes were carried out. The detection system for SBVgenotypes was optimized using each standard DNA and specific primer-pairs. Then PCR system for SBV-genotyping was applied with SBV-infected honeybee samples. Interestingly, in some of SBVinfected Apis mellifera, 2134D51 genotype (kSBV) and 2100D0 genotype (wSBV) were both detected, however, 2100D0 was existed dominantly. Whereas, 2100D0 was not detected from SBVinfected samples of Apis cerana, only 2134D51 was identified as solo pathogen in this species. PCR system for SBV-genotyping might be helpful to understand SBV-distribution and spreading.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
LITERATURE CITED
참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)] - Anderson, D. L. / 1988 / Inapparent virus infections and their interactions in the pupae of the honey bee (Apis mellifera Linnaeus) in Australia / Journal of General Virology 69 : 1617 ~ 1625
[학술지(정기간행물)] - Bailey, L. / 1964 / Sacbrood virus of the larval honey bee (Apis mellifera Linnaeus) / Virology 23 : 425 ~ 429
[학술지(정기간행물)] - Bailey, L. / 1972 / Effects of sacbrood virus on adult honey-bees / Annals of Applied Biology 72 : 27 ~ 35
[학술지(정기간행물)] - Bailey, L. / 1975 / Recent research on honey bee viruses / Bee World 56 : 55 ~ 64
[학술지(정기간행물)] - Choe, S. E. / 2012 / Analysis of the complete genome sequence of two Korean sacbrood viruses in the honey bee, Apis mellifera / Virology 432 : 155 ~
[학술지(정기간행물)] - Bailey, L. / 1964 / Sacbrood virus of the larval honey bee (Apis mellifera Linnaeus) / Virology 23 : 425 ~ 429
[학술지(정기간행물)] - Bailey, L. / 1972 / Effects of sacbrood virus on adult honey-bees / Annals of Applied Biology 72 : 27 ~ 35
[학술지(정기간행물)] - Bailey, L. / 1975 / Recent research on honey bee viruses / Bee World 56 : 55 ~ 64
[학술지(정기간행물)] - Choe, S. E. / 2012 / Analysis of the complete genome sequence of two Korean sacbrood viruses in the honey bee, Apis mellifera / Virology 432 : 155 ~
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UCI(KEPA) : I410-ECN-0101-2018-527-003142853