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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 293 - 298 (6page)
- DOI
- 10.7841/ksbbj.2018.33.4.293
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초록· 키워드
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Allium hookeri is distributed across Asia and is known to possess antioxidant and anti-inflammatory properties. AMPK is activated by ATP consumption or nutrient deficiency. It plays an important role as an energy sensor and apoptosis regulator. Moreover, Akt plays an important role in cell growth and division. It also regulates apoptosis-mediated proteins such as p53 and GSK-3β. GSK-3β, which is involved in growth metabolism, and various other transcription factors determine the survival or death of an organism. We therefore evaluated whether A. hookeri extract could inhibit cell proliferation and induce apoptosis through the AMPK/Akt/GSK-3β signaling pathway by performing MTT assay, western blotting, and Annexin V & Dead cell staining. MTT assay results revealed that AHE could effectively inhibit the AGS gastric cancer cells in dose-dependent manner (90, 135, 180, 225μg/ml) but not that of fibroblast cells (90, 135, 180, 225μg/ml). In other words, its action was unique to stomach cancer cells. Annexin V & Dead cell staining confirmed increase in the rate of apoptosis in a dose-dependent manner (90, 135, 180μg/ml). Furthermore, we identified that AHE decreased the expression of p-Akt, GSK-3β, b-catenin, pro caspase 3, and Bcl-2 anti-apoptotic proteins, while it increased those of p-AMPK, p53, cleaved PARP, cleaved caspase 3, and Bax pro-apoptotic proteins. Our cumulative results suggest that AHE decreases AGS cell proliferation and induces apoptosis by inhibiting Akt and AMPK expression.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (13)
참고문헌 신청
[학술지(정기간행물)] - Ayam, V. S. / 2011 / Allium hookeri, Thw. Enum. A lesser known terrestrial perennial herb used as food and its ethnobotanical relevance in Manipur / African J. Food Agric. Nutr. Development 11 : 5389 ~ 5412
[학술지(정기간행물)] - 김창현 / 2012 / 삼채 뿌리 메탄올 추출물이 LPS가 유도된 RAW264.7 세포에 대한 항염증 효과 / 한국식품영양과학회지 41 (11) : 1645 ~ 1648
[학술지(정기간행물)] - Buiatti, E. / 1989 / A case-control study of gastric cancer and diet in Italy / Int. J. Cancer 44 : 611 ~ 616
[학술지(정기간행물)] - Kahn, B. B. / 2005 / AMPactivated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism / Cell Metab 1 : 15 ~ 25
[학술지(정기간행물)] - Agarwal, S. / 2015 / AMPK control of mTORC1 is p53-and TSC2-independent in pemetrexed-treated carcinoma cells / J. Biol. Chem 290 : 27473 ~ 27486
[학술지(정기간행물)] - 김창현 / 2012 / 삼채 뿌리 메탄올 추출물이 LPS가 유도된 RAW264.7 세포에 대한 항염증 효과 / 한국식품영양과학회지 41 (11) : 1645 ~ 1648
[학술지(정기간행물)] - Buiatti, E. / 1989 / A case-control study of gastric cancer and diet in Italy / Int. J. Cancer 44 : 611 ~ 616
[학술지(정기간행물)] - Kahn, B. B. / 2005 / AMPactivated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism / Cell Metab 1 : 15 ~ 25
[학술지(정기간행물)] - Agarwal, S. / 2015 / AMPK control of mTORC1 is p53-and TSC2-independent in pemetrexed-treated carcinoma cells / J. Biol. Chem 290 : 27473 ~ 27486
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UCI(KEPA) : I410-ECN-0101-2019-470-000330288