지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Objectives: The objective of this study is Korean mistletoe lectin (Viscum album coloratum agglutinin, VCA) and bee venom (BV) to experimental prove comparative study of VCA and BV on the anti-cancer effect and mechanisms of action.
Methods: In this study, it was examined in a human hepatocellular carcinoma cell line, Hep G2 cells. Cytotoxic effects of VCA and BV on Hep G2 cells were determined by 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay in vitro. VCA and BV killed Hep G2 cells in a time- and dose-dependent manner.
Results: The apoptotic cell death was then confirmed by propidium iodide staining and DNA fragmentation analysis. The mechanisms of action was examined by the expression of anti-apoptotic proteins and activation of mitogen-activated protein kinases. Treatment of Hep G2 cells with VCA activated poly (ADP-ribose) polymerase-1 (PARP-1) known as a marker of apoptosis, and mitogen-activated protein kinases signaling pathways including SAPK/JNK, MAPK and p38. BV also activated PARP-1, MAPK, p38 but not JNK. The expression level of anti-apoptotic molecule, Bcl-X, was decreased by VCA treatment but not BV. Finally, the phosphorylation level of ERM proteins involved in the cytoskeleton homeostasis was decreased by both stimuli.
Conclusion: We examined the involvement of kinase in VCA or BV - induced apoptosis by using kinase inhibitors. VCA-induced apoptosis was partially inhibited by in the presence.
Methods: In this study, it was examined in a human hepatocellular carcinoma cell line, Hep G2 cells. Cytotoxic effects of VCA and BV on Hep G2 cells were determined by 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay in vitro. VCA and BV killed Hep G2 cells in a time- and dose-dependent manner.
Results: The apoptotic cell death was then confirmed by propidium iodide staining and DNA fragmentation analysis. The mechanisms of action was examined by the expression of anti-apoptotic proteins and activation of mitogen-activated protein kinases. Treatment of Hep G2 cells with VCA activated poly (ADP-ribose) polymerase-1 (PARP-1) known as a marker of apoptosis, and mitogen-activated protein kinases signaling pathways including SAPK/JNK, MAPK and p38. BV also activated PARP-1, MAPK, p38 but not JNK. The expression level of anti-apoptotic molecule, Bcl-X, was decreased by VCA treatment but not BV. Finally, the phosphorylation level of ERM proteins involved in the cytoskeleton homeostasis was decreased by both stimuli.
Conclusion: We examined the involvement of kinase in VCA or BV - induced apoptosis by using kinase inhibitors. VCA-induced apoptosis was partially inhibited by in the presence.
목차
Introduction
Material and Methods
Result
Result & Discussion
References
참고문헌 (25)
참고문헌 신청
[학술지(정기간행물)] - Ahn YO / 1989 / Incidence estimation of primary liver cancer among Koreans / Journal of Korean Cancer Association : 241 ~ 248
[보고서] - The Ministry of Health and Welfare / 2015 / the Korea Central Cancer Registration Center, and the annual report of Korea's Central Cancer Registration Project
[학술지(정기간행물)] - Stein GM / 1998 / Mistletoe in immunology and the clinic(short review) / Anticancer Research 18 : 3247 ~ 3249
[학술지(정기간행물)] - Stein GM / 1999 / Viscotoxin-free aqueous exyracts from European mistletoe (Viscum album L.) stimulate activity of human granulocytes / Anticancer Research 19 : 2925 ~ 2928
[학술지(정기간행물)] - Son DJ / 2007 / Therapeutic application of anti-arthritis, pain-releasing and anticancer effects of bee venom and its constituent compounds / Pharmacol Ther 115 (2) : 246 ~ 270
[보고서] - The Ministry of Health and Welfare / 2015 / the Korea Central Cancer Registration Center, and the annual report of Korea's Central Cancer Registration Project
[학술지(정기간행물)] - Stein GM / 1998 / Mistletoe in immunology and the clinic(short review) / Anticancer Research 18 : 3247 ~ 3249
[학술지(정기간행물)] - Stein GM / 1999 / Viscotoxin-free aqueous exyracts from European mistletoe (Viscum album L.) stimulate activity of human granulocytes / Anticancer Research 19 : 2925 ~ 2928
[학술지(정기간행물)] - Son DJ / 2007 / Therapeutic application of anti-arthritis, pain-releasing and anticancer effects of bee venom and its constituent compounds / Pharmacol Ther 115 (2) : 246 ~ 270
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Bee Venom Exerts Neuroprotective Effects on Neuronal Cells and Astrocytes under Hypoxic Conditions Through MAPK Signaling Pathways
Neonatal medicine
2016 .01
-
L-Ascorbic Acid Induces Apoptosis in Human Laryngeal Epidermoid Hep-2 Cells by Modulating the NF-κB/MAPK/Akt Signaling Pathway
International Journal of Oral Biology
2020 .01
-
Subcutaneous Injection of Bee Venom in Wistar Rats: effects on blood cells and biochemical parameters
Journal of Pharmacopuncture
2022 .09
-
Improved Therapeutic Profiles of PLA₂-Free Bee Venom Prepared by Ultrafiltration Method
Toxicological Research
2015 .03
-
Synergistic Effects of Bee Venom and Natural Killer Cells on B16F10 Melanoma Cell Growth Inhibition through IL-4-mediated Apoptosis
대한침구의학회지
2017 .01
-
Bee Venom (Apis Mellifera) an Effective Potential Alternative to Gentamicin for Specific Bacteria Strains
Journal of Pharmacopuncture
2016 .01
-
Honey Bee Venom (Apis mellifera) Contains Anticoagulation Factors and Increases the Blood-clotting Time
Journal of Pharmacopuncture
2015 .01
-
Molecular Mechanisms of Apoptosis and Roles in Cancer Development and Treatment
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Major Components of Clinically used Bee Venom Pharmacopuncture
대한침구의학회지
2017 .01
-
mRNA Expression of Bax, Bcl-2, p53, Cathepsin B, Caspase-3 and Caspase-9 in the HepG2 Cell Line Following Induction by a Novel Monoclonal Ab Hep88 mAb: Cross-Talk for Paraptosis and Apoptosis
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Cytotoxic Effect of Bee (A. mellifera) Venom on Cancer Cell Lines
Journal of Pharmacopuncture
2020 .01
-
Synergistic Effect of Natural Killer Cells and Bee Venom on Inhibition of NCI-H157 Cell Growth
대한침구의학회지
2016 .01
-
A Retrospective Study on the Clinical Safety of Bee Venom Pharmacopuncture at Craniofacial Acupuncture Points for the Treatment of Facial Disorders
대한침구의학회지
2019 .01
-
Antifungal Effects of Bee Venom Components on Trichophyton rubrum: A Novel Approach of Bee Venom Study for Possible Emerging Antifungal Agent
Annals of Dermatology
2018 .01
-
Viscum Album Var Hot Water Extract Mediates Anti-cancer Effects through G1 Phase Cell Cycle Arrest in SK-Hep1 Human Hepatocarcinoma cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Apoptosis Induction Effect of Zingiberis Rhizoma Extract in Microglia BV-2 Cells
International Journal of Oral Biology
2017 .01
-
항암화학요법 유발 말초신경병증에 대한 봉독 약침 요법의 효과 및 기전에 대한 실험연구 고찰
동의생리병리학회지
2021 .02
-
Isocryptotanshinone Induced Apoptosis and Activated MAPK Signaling in Human Breast Cancer MCF-7 Cells
Journal of Breast Cancer
2015 .01
-
Bee Venom Acupuncture Reduces Interleukin-6, Increases Interleukin-10, and Induces Locomotor Recovery in a Model of Spinal Cord Compression
Journal of Acupuncture & Meridian Studies
2017 .01
-
주사제 사용을 위한 봉독의 균질성 및 안정성 평가
한국가축위생학회지
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2019-519-000328659