지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 62 - 70 (9page)
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초록· 키워드
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Purpose: Bone marrow has long been a source of primary cells. This study was performed to evaluate the effects of age and sex on the cellular viability and expression of stem cell markers of mRNA and on the protein expression of bone marrow stem cells (BMSCs) derived from healthy donors.
Materials and Methods: Stem cells were isolated from human bone marrow and plated in culture plates. The shape of the BMSCs was observed under inverted microscope. Quantitative cellular viability was evaluated using a Cell-Counting Kit-8 assay. The expression of stem cell surface markers was tested and a series of quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot was performed to evaluate the expression in each group.
Result: The shapes of the cells at 20s, 30s, and 50s were similar to each other. No significant changes in cellular viability were noted among different age groups or sex groups. The BMSCs expressed CD44, CD73, and CD90 surface markers but did not express CD14 and CD34. There were no noticeable differences in CD surface markers among the different age groups. The expressions of CD surface markers were similar between men and women. No significant differences in the secretion of vascular endothelial growth factors (VEGFs) were noted at Day 3 between different age groups. qRT-PCR regarding the expression showed differences between the age groups. However, Western blot analysis showed a decrease in expression but did not reach statistical significance (P>0.05).
Conclusion: This study clearly showed no significant differences in shape, cell viability, expression of stem cell surface markers, or secretion of human VEGF among different age groups. However, western blot analysis showed a tendency of age-related decrease which did not reach statistical significance. Collectively, autologous or allogeneic BMSCs should be meticulously applied to obtain optimal results regarding age and sex.
Materials and Methods: Stem cells were isolated from human bone marrow and plated in culture plates. The shape of the BMSCs was observed under inverted microscope. Quantitative cellular viability was evaluated using a Cell-Counting Kit-8 assay. The expression of stem cell surface markers was tested and a series of quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot was performed to evaluate the expression in each group.
Result: The shapes of the cells at 20s, 30s, and 50s were similar to each other. No significant changes in cellular viability were noted among different age groups or sex groups. The BMSCs expressed CD44, CD73, and CD90 surface markers but did not express CD14 and CD34. There were no noticeable differences in CD surface markers among the different age groups. The expressions of CD surface markers were similar between men and women. No significant differences in the secretion of vascular endothelial growth factors (VEGFs) were noted at Day 3 between different age groups. qRT-PCR regarding the expression showed differences between the age groups. However, Western blot analysis showed a decrease in expression but did not reach statistical significance (P>0.05).
Conclusion: This study clearly showed no significant differences in shape, cell viability, expression of stem cell surface markers, or secretion of human VEGF among different age groups. However, western blot analysis showed a tendency of age-related decrease which did not reach statistical significance. Collectively, autologous or allogeneic BMSCs should be meticulously applied to obtain optimal results regarding age and sex.
목차
Introduction
Materials and Methods
Result
Discussion
Conclusion
References
참고문헌 (11)
참고문헌 신청
[학술지(정기간행물)] - Peptanariu D / 2012 / Optimization of culture conditions for bone marrow stromal cells in RPMI-1640 medium / Rev Med Chir Soc Med Nat Iasi 116 : 222 ~ 227
[학술지(정기간행물)] - Phinney DG / 1999 / Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation / J Cell Biochem 72 : 570 ~ 585
[학술지(정기간행물)] - Bianco P / 2001 / Bone marrow stromal stem cells: nature, biology, and potential applications / Stem Cells 19 : 180 ~ 192
[학술지(정기간행물)] - Deans RJ / 2000 / Mesenchymal stem cells: biology and potential clinical uses / Exp Hematol 28 : 875 ~ 884
[학술지(정기간행물)] - Pereira RF / 1995 / Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice / Proc Natl Acad Sci U S A 92 : 4857 ~ 4861
[학술지(정기간행물)] - Phinney DG / 1999 / Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation / J Cell Biochem 72 : 570 ~ 585
[학술지(정기간행물)] - Bianco P / 2001 / Bone marrow stromal stem cells: nature, biology, and potential applications / Stem Cells 19 : 180 ~ 192
[학술지(정기간행물)] - Deans RJ / 2000 / Mesenchymal stem cells: biology and potential clinical uses / Exp Hematol 28 : 875 ~ 884
[학술지(정기간행물)] - Pereira RF / 1995 / Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice / Proc Natl Acad Sci U S A 92 : 4857 ~ 4861
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