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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 429 - 434 (6page)
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이 논문의 연구 히스토리 (4)
2014
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This study describes the efficient method for the discrimination of ‘Cheonryang’ in Panax ginseng Meyerusing a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminatingKorean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019,MFGp 0248, and successful identification of ‘Cheonryang’ was achieved from out of 11 Korean ginseng cultivars. Two differentsizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the samesize of amplified DNAs (389 bp), but ‘Cheonryang’ showed a different size. Thus ‘Cheonryang’ can be efficiently distinguishedfrom the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizesof DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shownin five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified inthe other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of ‘Cheonryang’ cultivar from alarge number of samples.
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참고문헌 (25)
참고문헌 신청
Bang KH / 2010 / Construction of genomic DNA library of Korean ginseng(Panax ginseng C. A. Meyer)and development of sequence-tagged sites / Biological and Pharmaceutical Bulletin 33:1579~1588
방경환 / 2011 / SSR 마커를 이용한 고려인삼 품종 판별기술 개발 / 한국약용작물학회지 19(3):185~190
Bernet GP / 2003 / Applicability of molecular markers in the context of the protection of new varieties of cucumber / Plant Breeding 122:146~152
Blake TK / 1996 / STS-PCR markers appropriate for wheat-barley introgression / Theoretical and Applied Genetics 93:826~832
Cooke RJ / 2003 / Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci / Euphytica 132:331~341
방경환 / 2011 / SSR 마커를 이용한 고려인삼 품종 판별기술 개발 / 한국약용작물학회지 19(3):185~190
Bernet GP / 2003 / Applicability of molecular markers in the context of the protection of new varieties of cucumber / Plant Breeding 122:146~152
Blake TK / 1996 / STS-PCR markers appropriate for wheat-barley introgression / Theoretical and Applied Genetics 93:826~832
Cooke RJ / 2003 / Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci / Euphytica 132:331~341
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UCI(KEPA) : I410-ECN-0101-2019-480-000587996