지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Influenza A (H1N1) virus caused a worldwide pandemic in 2009–2010 and still remains in seasonal circulation. Continuous surveillance activities are encouraged in the post pandemic phase to watch over the trend of occurrence every year, this is better to be done by a rapid and sensitive method for its detection. This study was conducted to detect proportions of occurrence of influenza A virus (H1N1) in patients with influenza-like illness. Samples from 500 patients with influenza or influenza-like clinical presentation were tested by real-time reverse transcription polymerase chain reaction (RT-PCR) and virus tissue culture. Among the total 500 participants, 193 (38.6%) were females and 307 (61.4%) males. Seventy-one patients (14.2%) were positive for H1N1 virus infection with real-time RT-PCR while 52 (10.4%) were positive by tissue culture. Non-statistically significant relation was found between age and gender with the positivity of H1N1. Sensitivity and specificity of real-time RT-PCR was 98.08% and 95.54%, respectively, in comparison to virus isolation with accuracy 95.8%. This study showed that H1N1 virus was responsible for a good proportion of influenza during the post-pandemic period. Real-time RT-PCR provides rapidity and sensitivity for the detection of influenza A virus (H1N1) compared with virus isolation and thus it is recommended as a diagnostic tool.
목차
Materials and Methods
Results
Discussion
Conclusion
References
참고문헌 (51)
참고문헌 신청
Amaravathi KS, Sakuntala P, Sudarsi B, Manohar S, Nagamani R, and Rao SR. 2015. Clinical profile and outcome of recent outbreak of influenza A H1N1 (swine flu) at a tertiary care center in Hyderabad, Telangana. Ann. Trop. Med. Public Health 8, 267? 271.
Angione SL, Inde Z, Beck CM, Artenstein AW, Opal SM, and Tripathi A. 2013. Microdroplet sandwich real-time RT-PCR for detection of pandemic and seasonal influenza subtypes. PLoS One 8, e73497.
Behzadi MA, Ziyaeyan M, and Alborzi A. 2016. A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A. Arch. Med. Sci. 12, 1286?1292.
Centers for Disease Control and Prevention. 2002. Prevention and control of influenza. Morb. Mortal. Wkly. Rep. 51, 1?31.
Chauhan N, Narang J, Pundir S, Singh S, and Pundir CS. 2013. Laboratory diagnosis of swine flu: a review. Artif. Cells Nanomed. Biotechnol. 41, 189?195.
Angione SL, Inde Z, Beck CM, Artenstein AW, Opal SM, and Tripathi A. 2013. Microdroplet sandwich real-time RT-PCR for detection of pandemic and seasonal influenza subtypes. PLoS One 8, e73497.
Behzadi MA, Ziyaeyan M, and Alborzi A. 2016. A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A. Arch. Med. Sci. 12, 1286?1292.
Centers for Disease Control and Prevention. 2002. Prevention and control of influenza. Morb. Mortal. Wkly. Rep. 51, 1?31.
Chauhan N, Narang J, Pundir S, Singh S, and Pundir CS. 2013. Laboratory diagnosis of swine flu: a review. Artif. Cells Nanomed. Biotechnol. 41, 189?195.
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