지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Lysyl oxidase (LOX), extracellular matrix enzyme, is catalyzing lysine-derived crosslinks in collagen and elastin. Recently, several LOX-like proteins (LOXL, LOXL2, LOXL3 and LOXL4) have been identified in human but their specific functions are still largely unknown. The purpose of this study was to evaluate the function of the LOX family genes during odontoblastic differentiation of human dental pulp (HDP) cells induced with odontogenic supplement (OS). The messenger RNA (mRNA) expression of LOX family genes and differentiation markers was assessed by reverse transcriptase polymerase chain reaction analysis (RT-PCR). The formation of mineralization nodules was evaluated by alrizarin red S staining. Amine oxidase activity of HDP cells was measured by peroxidase-coupled fluormetric assay. The expressions of differentiation markers, such as alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), dentin matrix protein1 (DMP1), dentin sialophosphoprotein (DSPP) in HDP cells were increased after treatment with OS media. The LOX and LOXL mRNA expression were gradually increased in OS media, whereas LOX enzyme activities were markedly detected on day 7. The mRNA expression and LOX enzyme activity of collagen type I was very similar to the pattern of LOX gene. In this study, the expression of LOX and its isoforms, and activity of LOX were highly regulated during odontoblastic differentiation. Thus, these results suggest that LOX plays a key role in odontoblastic differentiation of HDP cells.
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참고문헌 (25)
참고문헌 신청
About I / 2000 / Human dentin production in vitro / Exp Cell Res 258 : 33 ~ 41
Atsawasuwan P / 2005 / Expression of lysyl oxidase isoforms in MC3T3-E1 osteoblastic cells / Biochem Biophys Res Commun 327 : 1042 ~ 1046
Butler WT / 1995 / The nature and functional significance of dentin extracellular matrix proteins / Int J Dev Biol 39 : 169 ~ 179
Chang SW / 2009 / Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells / J Endod 35 : 1222 ~ 1228
Chen S / 2005 / Differential regulation of dentin sialophosphoprotein expression by Runx2 during odontoblast cytodifferentiation / J Biol Chem 280 : 29717 ~ 29727
Atsawasuwan P / 2005 / Expression of lysyl oxidase isoforms in MC3T3-E1 osteoblastic cells / Biochem Biophys Res Commun 327 : 1042 ~ 1046
Butler WT / 1995 / The nature and functional significance of dentin extracellular matrix proteins / Int J Dev Biol 39 : 169 ~ 179
Chang SW / 2009 / Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells / J Endod 35 : 1222 ~ 1228
Chen S / 2005 / Differential regulation of dentin sialophosphoprotein expression by Runx2 during odontoblast cytodifferentiation / J Biol Chem 280 : 29717 ~ 29727
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