지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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The aim of this study was to evaluate influences of titanium dioxide (TiO2) concentrations and irradiation times on growth of Streptococcus mutanswhen irradiated by visible light (405 nm wavelength) and by ultraviolet light (254 nm wavelength). To find the optimal antibacterial concentration ofTiO2, 0.01, 0.1, 1.0, and 10.0 mg/ml TiO2 suspension was prepared with sterilized distilled water. S. mutans cultured media was added to TiO2solution to set the final cell count to 104 CFU/ml. The photocatalytic reaction was induced by irradiating 254 nm and 405 nm lights for 10 minutes.
To compare the bactericidal activities according to irradiation times, all photocatalytic reaction was carried out with 0.1 mg/ml TiO2 for 0, 10, 20,30, and 40 minutes with both lights. After the photocatalytic reaction, 100 μm of the reaction mixture was immediately plated on brain heart infusionagar. These plates were placed at 5% CO2, 37oC, for 24 hours and the bacterial colonies were counted. All experiments were performed inquintuplicate. One-way ANOVA was used to determine whether there were any significant differences between the TiO2 concentrations or theirradiation times. The most effective concentration of TiO2 for its photocatalytic bactericidal effect on S. mutans was 0.1 mg/ml when irradiated with254 nm and 405 nm lights. The longer the irradiation time, the bigger the bactericidal effect for both wavelengths. Over 99% of bacteria in theinoculum were killed after irradiation with 254 nm for 20 minutes and with 405 nm for 40 minutes. In conclusion, a photocatalytic reaction of TiO2induced by visible light of 405 nm constitutes the bactericidal effect on S. mutans.
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참고문헌 (22)
참고문헌 신청
Chen J / 2002 / New technology for deep light distribution in tissue for phototherapy / Cancer J 8 : 154 ~ 163
Choi J: / 2005 / Photocatalytic antibacterial effect of TiO2 film of TiAg on cariogenic bacterias / doctor / Yonsei University
Flotra L, Gjermo P / 1971 / Side effects of chlorhexidine mouth washes / Scand J Dent Res 79 : 119 ~ 125
Fujishima A / 2000 / Titanium dioxide photocatalysis / J Photochem Photobiol C Photochem Rev 1 : 1 ~ 21
Gao Y / 2013 / The effects of different wavelength UV photofunctionalization on micro-arc oxidized titanium / PLoS One 8 : e68086 ~
Choi J: / 2005 / Photocatalytic antibacterial effect of TiO2 film of TiAg on cariogenic bacterias / doctor / Yonsei University
Flotra L, Gjermo P / 1971 / Side effects of chlorhexidine mouth washes / Scand J Dent Res 79 : 119 ~ 125
Fujishima A / 2000 / Titanium dioxide photocatalysis / J Photochem Photobiol C Photochem Rev 1 : 1 ~ 21
Gao Y / 2013 / The effects of different wavelength UV photofunctionalization on micro-arc oxidized titanium / PLoS One 8 : e68086 ~
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