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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 323 - 328 (6page)
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We screened four B. thuringiensis strains whose parasporal inclusions contained the S-layer protein (SLP), and cloned two slp genes from each strain. Phylogenetic analysis indicated these SLPs could be divided into two groups, SLP1s and SLP2s.
To confirm whether SLPs were present in the S-layer or as a parasporal inclusion, strains CTC and BMB1152 were chosen for further study. Western blots with isolated S-layer proteins from strains CTC and BMB1152 in the vegetative phase showed that SLP1s and SLP2s were constituents of the S-layer.
Immunofluorescence utilizing spore-inclusion mixtures of strains CTC and BMB1152 in the sporulation phase showed that SLP1s and SLP2s were also constituents of parasporal inclusions. When heterogeneously expressed in the crystal negative strain BMB171, four SLPs from strains CTC and BMB1152 could also form parasporal inclusions. This temporal and spatial expression is not an occasional phenomenon but ubiquitous in B. thuringiensis strains. [BMB reports 2011;44(5): 323-328]
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참고문헌 (24)
참고문헌 신청
Ahn, J. S / 2006 / Characterization of CidR-mediated regulation in Bacillus anthracis reveals a previously undetected role of S-layer proteins as murein hydrolases / Mol. Mi
Beveridge, T. J / 1997 / Functions of S-layers / FEMS Microbiol. Rev 208 : 99 ~ 149
Guo, G / 2008 / A new group of parasporal inclusions encoded by the S-layer gene of Bacillus thuringiensis / FEMS Microbiol. Lett 282 : 1 ~ 7
Jarosch, M / 2001 / Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms / Micr
Konstantinov, S. R / 2008 / S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions / Proc. Natl. Acad. Sci. U.S.A 105 : 1
Beveridge, T. J / 1997 / Functions of S-layers / FEMS Microbiol. Rev 208 : 99 ~ 149
Guo, G / 2008 / A new group of parasporal inclusions encoded by the S-layer gene of Bacillus thuringiensis / FEMS Microbiol. Lett 282 : 1 ~ 7
Jarosch, M / 2001 / Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms / Micr
Konstantinov, S. R / 2008 / S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions / Proc. Natl. Acad. Sci. U.S.A 105 : 1
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