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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 726 - 731 (6page)
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We report the tissue-specific distribution of chitinolytic activity in Korean ginseng root and characterize two 31-kDa chitinolytic enzymes. These two enzymes (SBF1 and SBF2) were purified 70- and 81-fold with yields of 0.75 and 1.25%, respectively,and exhibited optimal pH and temperature ranges of 5.0-5.5 and 40-50℃. With [3H]-chitin as a substrate, Km and Vmax values of SBF1 were 4.6 mM and 220 mmol/mg-protein/h, respectively, while those of SBF2 were 7.14 mM and 287mmol/mg-protein/h. The purified enzymes showed markedly less activity with p-nitrophenyl-N-acetylglucosaminide and fluorescent 4-methylumbelliferyl glycosides of D-N-acetylglucosamine oligomers than with [3H]-chitin. End-product inhibition of both enzymes demonstrated that both are endochitinases with different N-acetylglucosaminidase activity. Furthermore,the NH2-terminal sequence of SBF1 showed a high degree of homology with other plant chitinases whereas the NH2-terminal amino acid of SBF2 was blocked.
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참고문헌 (22)
참고문헌 신청
Baek, J. H. / 2001 / Distribution of chitinases in rice (Oryza sativa L) seed and characterization of a hull-specific chitinase / J. Biochem. Molec. Biol. 34 : 310 ~ 315
Chen, Z. K. / 1998 / Isolation and characterization of a group of oligopeptides related to oxidized glutathione from the root of Panax ginseng / J. Pept. Res. 52 : 137 ~ 142
Emanuelsson, O. / 2007 / Locating proteins in the cell using TargetP, SignalP and related tools / Nature Protocols 2 : 953 ~ 971
Han, B. K. / 2000 / Purification and characterization of acidic chitinases from gizzards of broiler (Gallus gallus L.) / J. Biochem. Molec. Biol. 33 : 326 ~ 331
Kajiwara, H. / 1996 / Evidence of metal binding activities of pentadecapeptide from Panax ginseng / J. Chromatogr. B Biomed. 687 : 443 ~ 448
Chen, Z. K. / 1998 / Isolation and characterization of a group of oligopeptides related to oxidized glutathione from the root of Panax ginseng / J. Pept. Res. 52 : 137 ~ 142
Emanuelsson, O. / 2007 / Locating proteins in the cell using TargetP, SignalP and related tools / Nature Protocols 2 : 953 ~ 971
Han, B. K. / 2000 / Purification and characterization of acidic chitinases from gizzards of broiler (Gallus gallus L.) / J. Biochem. Molec. Biol. 33 : 326 ~ 331
Kajiwara, H. / 1996 / Evidence of metal binding activities of pentadecapeptide from Panax ginseng / J. Chromatogr. B Biomed. 687 : 443 ~ 448
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