지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 135 - 136 (2page)
이용수
초록· 키워드
오류제보하기
Small interfering RNAs (siRNAs) have been developed to intentionally repress a specific gene expression by directing RNA-induced silencing complex (RISC), mimicking the endogenous gene silencer, microRNAs (miRNAs). Although siRNA is designed to be perfectly complementary to an intended target mRNA, it also suppresses hundreds of off-targets by the way that miRNAs recognize targets. Until now, there is no efficient way to avoid such off-target repression, although the mode of miRNA-like interaction has been proposed. Rationally based on the model called “transitional nucleation” which pre-requires base-pairs from position 2 to the pivot (position 6) with targets, we developed a simple chemical modification which completely eliminates miRNA-like off-target repression (0%), achieved by substituting a nucleotide in pivot with abasic spacers (dSpacer or C3 spacer), which potentially destabilize the transitional nucleation. Furthermore, by alleviating steric hindrance in the complex with Argonaute (Ago), abasic pivot substitution also preserves near-perfect on-target activity (∼80-100%). Abasic pivot substitution offers a general means of harnessing target specificity of siRNAs to experimental and clinical applications where misleading and deleterious phenotypes from off-target repression must be considered.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Efficient generation of human induced pluripotent stem cells using self-assembled siRNA/miRNA nanoparticles
한국생물공학회 학술대회
2018 .10
-
Development of Novel Cancer Growth-inhibiting siRNA for Type-specific Breast Cancer Targeted Therapy
한국생물공학회 학술대회
2022 .04
-
New paradigms on siRNA local application
BMB Reports
2015 .01
-
MMPs-responsive release of siRNA from 4-arm PEG-siRNA conjugate
한국고분자학회 학술대회 연구논문 초록집
2015 .10
-
EphB4 Targeted Liposomal siRNA System to Induce Selective siRNA Delivery for Ovarian Carcinoma
한국고분자학회 학술대회 연구논문 초록집
2018 .10
-
Targeted Delivery of siRNAs to Breast Cancer Cells by Fusion Peptides
한국생물공학회 학술대회
2020 .10
-
Rules for functional microRNA targeting
BMB Reports
2017 .01
-
Synthesis of Dual-targeting Nanoparticles for Polymeric siRNA Delivery to HER2 and CD44 Overexpressed Cancer Cell
한국생물공학회 학술대회
2019 .10
-
Novel Fusion Peptide-mediated Self-assembled Complexes for Enhanced siRNA Delivery
한국생물공학회 학술대회
2019 .10
-
타겟 유전자 기반 miRNA 네트워크를 통한 질병 관련 miRNA 추출 방법
한국정보기술학회논문지
2015 .01
-
Enhanced siRNA Delivery via Fusion Peptide-mediated Self-assembly Complex
한국생물공학회 학술대회
2019 .04
-
siRNA와 항암제의 동시전달을 위한 나노전달체 개발
고분자 과학과 기술
2017 .06
-
A novel anti-VEGF siRNA delivery system for the treatment of AMD model
한국생물공학회 학술대회
2016 .04
-
Enhanced siRNA Skin Delivery via Peptide-Assisted Self-Structure
한국생물공학회 학술대회
2018 .10
-
Non-canonical targets play an important role in microRNA stability control mechanisms
BMB Reports
2017 .01
-
Targeted Delivery of siRNAs through Nanocomplexs with Novel Fusion Peptides for Breast Cancer Treatment
한국생물공학회 학술대회
2022 .04
-
Fabrication of Nano-complex Composed with siRNA and Au Nanorods for Cancer Therapy
한국생물공학회 학술대회
2016 .04
-
Robust gold-installed nanocomplexes as siRNA carriers for enhanced tumor targeting
한국고분자학회 학술대회 연구논문 초록집
2015 .04
-
Study for efficient gene therapy via siRNA-ligand conjugates
한국고분자학회 학술대회 연구논문 초록집
2015 .04
-
A New Method for Uracil DNA Glycosylase Assay based on Abasic Site-Directed Nicking Enzyme Suppression
한국생물공학회 학술대회
2017 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0