지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 521 - 525 (5page)
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In addition to its pivotal role in neuronal survival, PI3K/Akt signaling is integral to neuronal differentiation and neurite outgrowth. However, the exact role of Akt in neuronal differentiation is still controversial. Here, we found that nuclear expression of CA-Akt resulted in unusual rapid neurite outgrowth and overexpression of KD-Akt caused multiple dendrite growth without specific axon elongation. Moreover,microarray data revealed that the expression of FOXQ1expression was about 10-fold higher in cells with nuclear,active Akt than in control cells. Quantitative real-time PCR analysis showed that mRNA levels were upregulated in NLS-CA-Akt cells as compared to KD or EV cells. Furthermore,our FACS analysis demonstrated that overexpression of NLS-CA-Akt accumulate cells in the G1 phase within 24 h,fitting with the rapid sprouting of neuritis. Thus, our data implied that at least in this early time frame, the overexpression of nuclear, active Akt forced cells into neurite development through probably FOXQ1regulation. [BMB Reports 2012; 45(9): 521-525]
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참고문헌 (23)
참고문헌 신청
Ahn, J. Y. / 2004 / PIKE/nuclear PI 3-kinase signaling mediates the antiapoptotic actions of NGF in the nucleus / EMBO J. 23 : 3995 ~ 4006
Ahn, J. Y. / 2006 / Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase / EMBO J. 25 : 2083 ~ 2095
Ashcroft, M. / 1999 / The selective and inducible activation of endogenous PI 3-kinase in PC12 cells results in efficient NGF-mediated survival but defective neurite outgrow
Bang, O. S. / 2001 / Overexpression of Akt inhibits NGF-induced growth arrest and neuronal differentiation of PC12 cells / J. Cell. Sci. 114 : 81 ~ 88
Brunet, A. / 2001 / Transcription-dependent and -independent control of neuronal survival by the PI3K-Akt signaling pathway / Curr. Opin. Neurobiol. 11 : 297 ~ 305
Ahn, J. Y. / 2006 / Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase / EMBO J. 25 : 2083 ~ 2095
Ashcroft, M. / 1999 / The selective and inducible activation of endogenous PI 3-kinase in PC12 cells results in efficient NGF-mediated survival but defective neurite outgrow
Bang, O. S. / 2001 / Overexpression of Akt inhibits NGF-induced growth arrest and neuronal differentiation of PC12 cells / J. Cell. Sci. 114 : 81 ~ 88
Brunet, A. / 2001 / Transcription-dependent and -independent control of neuronal survival by the PI3K-Akt signaling pathway / Curr. Opin. Neurobiol. 11 : 297 ~ 305
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